Rat Anti-IFNG Recombinant Antibody (clone 9D7) (CAT#: PABW-080)

Figure 1 Effects of IL-10 on the expression of CD54 (ICAM-1), HLA-DR (MHC class II), MHC class I, CD80 (B7-1), CD86 (B7-2), and CD58 (LFA-3) Ags by human monocytes.

Human monocytes, isolated by negative selection using magnetic beads, were stained with the different mAbs either immediately (straight lines) or following incubation in medium alone (large lines) or in the presence of IL-10 (100 U/ml; dotted lines) for 72 h and then analyzed for the expression of MHC class I (W6/32), HLA-DR (L243), CD54 (ICAM-1-LB-2), CD80 (L307), CD86 (IT2.2), or CD58 (L308) Ags.

Groux, H., Bigler, M., De Vries, J. E., & Roncarolo, M. G. (1998). Inhibitory and stimulatory effects of IL-10 on human CD8+ T cells. The Journal of Immunology, 160(7), 3188-3193.

Figure 2 IL-10 act as a cofactor for IL-2 to induce CD8+ T-cell proliferation.

A, Purified CD8+ T cells were stimulated with cross-linked anti-CD3 mAb alone (gray bars) or in the presence of anti-IL-2 mAb (BG-5, 10 μg/ml; white stripped bars) or anti-IL-2 R α-chain (B-B10, 10 μg/ml; black bars) or both anti-IL-2 and anti-IL-2R α-chain (white bars). Where indicated, IL-10 was added at 100 U/ml and anti-IL-10 (9D7) at 10 μg/ml. B, Purified CD8+ T cells were stimulated with cross-linked anti-CD3 mAbs. After 5 days, cells were collected, washed, and cultured with different concentrations of IL-10 either alone (○) or in the presence of 2 U/ml (▪ or 20 U/ml (♦) of rIL-2. Cell proliferation was measured by a pulse of [3H]TdR in the last 12 h of day 2. No IL-2 secretion was detectable by ELISA on preactivated CD8+ T cells.

Groux, H., Bigler, M., De Vries, J. E., & Roncarolo, M. G. (1998). Inhibitory and stimulatory effects of IL-10 on human CD8+ T cells. The Journal of Immunology, 160(7), 3188-3193.