Human Anti-IL13 Recombinant Antibody (clone CNTO607) (CAT#: PABW-170)

Recombinant Human Antibody (CNTO607) is capable of binding to IL13, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IL13 mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti- IL13 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. CNTO607 binds human IL-13 with high affinity (Kd=15 pM) and prevents binding of IL-13 to both IL-13Rα1 and IL- 13Rα2. CNTO607 potently neutralizes the activity of IL-13 in a variety of bioassays, including cell survival, eotaxin production, and STAT6 signaling.


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ELISA

Figure 1 Anti-IL13 Recombinant Antibody (PABW-170) in ELISA

Figure 1 Anti-IL13 Recombinant Antibody (PABW-170) in ELISA

ELISA analysis of PABW-170 was performed by coating with human IL13 protein (His tag). Then blocked with BSA and incubated with PABW-170. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

WB

Figure 2 Anti-IL13 Recombinant Antibody (PABW-170) in WB

Figure 2 Anti-IL13 Recombinant Antibody (PABW-170) in WB

Western blot analysis of PABW-170 was performed by loading human IL13 protein (Lane 1 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABW-170 and a HRP goat anti-human IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

DB

Figure 3 Anti-IL13 Recombinant Antibody (PABW-170) in DB

Figure 3 Anti-IL13 Recombinant Antibody (PABW-170) in DB

Dot Blot analysis of PABW-170 was performed by coating with human IL13 protein (His tag).
PABW-170 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control


Specifications

  • Immunogen
  • Human interleukin 13
  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Human IL13
  • Species Reactivity
  • Human
  • Clone
  • CNTO607
  • Applications
  • ELISA, WB, Neut

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • IL13; interleukin 13; P600; IL-13; interleukin-13

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone CNTO607"

See other products for "IL13"

Recombinant Antibody

CAT Product Name Application Type
PABL-232 Human Anti-IL13 Recombinant Antibody (clone M1295) WB, IHC, FuncS Human IgG
HPAB-0226CQ Human Anti-IL13 Recombinant Antibody (HPAB-0226CQ) ELISA, Neut, FC, FuncS Human IgG2
HPAB-0227CQ Human Anti-IL13 Recombinant Antibody (HPAB-0227CQ) ELISA, Neut, FC, FuncS Human IgG2
HPAB-0228CQ Mouse Anti-IL13 Recombinant Antibody (clone MJ2-7) ELISA, FC, Neut, FuncS Mouse IgG
HPAB-0229CQ Human Anti-IL13 Recombinant Antibody (clone huMJ2-7) ELISA, FC, DB, Neut, FuncS Humanized IgG1, κ

Chimeric Antibody

CAT Product Name Application Type
TAB-094ZJ Anti-Human IL-13 Recombinant Antibody (167) ELISA Chimeric antibody (mouse/human)
TAB-094ZJ-S(P) Anti-Human IL-13 Recombinant Antibody scFv Fragment (167) ELISA Chimeric antibody (mouse/human)
TAB-094ZJ-F(E) Anti-Human IL-13 Recombinant Antibody Fab Fragment (167) ELISA Chimeric antibody (mouse/human)

Rabbit Monoclonal Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H05 Afuco™ Anti-IL13 ADCC Recombinant Antibody (Anrukinzumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody
AFC-TAB-017ML Afuco™ Anti-IL13 ADCC Recombinant Antibody (Dectrekumab), ADCC Enhanced ELISA, IHC, FC, IP, IF, Inhib ADCC enhanced antibody
AFC-TAB-219 Afuco™ Anti-IL13 ADCC Recombinant Antibody (Lebrikizumab), ADCC Enhanced ELISA, Neut, IF, IP, FC, FuncS ADCC enhanced antibody
AFC-TAB-218 Afuco™ Anti-IL13 ADCC Recombinant Antibody (Tralokinumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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