Human Anti-IL13 Recombinant Antibody (clone CNTO607)
CAT#: PABW-170
Recombinant Human Antibody (CNTO607) is capable of binding to IL13, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IL13 mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti- IL13 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. CNTO607 binds human IL-13 with high affinity (Kd=15 pM) and prevents binding of IL-13 to both IL-13Rα1 and IL- 13Rα2. CNTO607 potently neutralizes the activity of IL-13 in a variety of bioassays, including cell survival, eotaxin production, and STAT6 signaling.
Tested Data
Gene Expression
ELISA
Figure 1 Anti-IL13 Recombinant Antibody (PABW-170) in ELISA
ELISA analysis of PABW-170 was performed by coating with human IL13 protein (His tag). Then blocked with BSA and incubated with PABW-170. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-IL13 Recombinant Antibody (PABW-170) in WB
Western blot analysis of PABW-170 was performed by loading human IL13 protein (Lane 1 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABW-170 and a HRP goat anti-human IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-IL13 Recombinant Antibody (PABW-170) in DB
Dot Blot analysis of PABW-170 was performed by coating with human IL13 protein (His tag).
PABW-170 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
SDS-PAGE
Figure 4 Human Anti-IL13 Recombinant Antibody (clone CNTO607) (PABW-170) in SDS-PAGE
SDS-PAGE analysis of PABW-170 in non-reduced and reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 5 Human Anti-IL13 Recombinant Antibody (clone CNTO607) (PABW-170) in SEC-HPLC
SEC-HPLC analysis of PABW-170 shows that the purity is more than 95%.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42421/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42421/475_E8_1_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42421/477_E8_1_blue_red_green.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enriched (HDLM-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169194-IL13
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Specifications
- Immunogen
- Human interleukin 13
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human IL13
- Species Reactivity
- Human
- Clone
- CNTO607
- Applications
- ELISA, WB, Neut
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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(Creative Biolabs Cat# PABW-170, RRID: AB_3111690)
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Related Diseases
Asthma
Inflammatory Bowel Diseases
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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