Human Anti-IL13 Recombinant Antibody (clone CNTO607) (CAT#: PABW-170)

Figure 1 Anti-IL13 Recombinant Antibody (PABW-170) in ELISA

ELISA analysis of PABW-170 was performed by coating with human IL13 protein (His tag). Then blocked with BSA and incubated with PABW-170. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-IL13 Recombinant Antibody (PABW-170) in WB

Western blot analysis of PABW-170 was performed by loading human IL13 protein (Lane 1 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABW-170 and a HRP goat anti-human IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 3 Anti-IL13 Recombinant Antibody (PABW-170) in DB

Dot Blot analysis of PABW-170 was performed by coating with human IL13 protein (His tag).
PABW-170 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control

Figure 4 Human Anti-IL13 Recombinant Antibody (clone CNTO607) (PABW-170) in SDS-PAGE

SDS-PAGE analysis of PABW-170 in non-reduced and reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 5 Human Anti-IL13 Recombinant Antibody (clone CNTO607) (PABW-170) in SEC-HPLC

SEC-HPLC analysis of PABW-170 shows that the purity is more than 95%.