Rat Anti-IL18 Recombinant Antibody (clone 2C10); scFv Fragment (CAT#: HPAB-0067-YC-S(P))
Interleukin-18 (IL-18) is a potent cytokine that plays a role in both innate and acquired immune responses. IL-18 was first identified for its ability to induce the production of IFN-ɣ, one of the primary effector molecules of a T helper (Th) 1-type immune response. Anti-IL-18 antibodies may be useful for example, in diagnosing and treating disorders associated with elevated IL-18 levels, including inflammatory, cardiovascular and autoimmune diseases
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC-S(P)) in DB
Dot Blot analysis of HPAB-0067-YC-S(P) was performed by coating with human IL18 protein (His tag).
HPAB-0067-YC-S(P) incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-His tag as a secondary antibody (1: 2000)
Figure 2 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC-S(P)) in WB
Western blot analysis of HPAB-0067-YC-S(P) was performed by loading human IL18 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0067-YC-S(P) and HRP goat anti-His tag as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Figure 3 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC-S(P)) in ELISA
ELISA analysis of HPAB-0067-YC-S(P) was performed by coating with human IL18 protein (His tag). Then blocked with BSA and incubated with HPAB-0067-YC-S(P). The HRP-conjugated goat anti-His tag as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- Recombinant IL-18
- Host Species
- Rat
- Type
- Rat scFv
- Specificity
- Human IL18
- Species Reactivity
- Human
- Clone
- 2C10
- Applications
- Neut
- Related Disease
- Inflammatory, Cardiovascular, Autoimmune diseases
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0373CL | Human Anti-IL18 Recombinant Antibody (TAB-0373CL) | ELISA, FuncS, Inhib | Human IgG |
TAB-0374CL | Human Anti-IL18 Recombinant Antibody (TAB-0374CL) | ELISA, FuncS, Inhib | Human IgG |
TAB-0373CL-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (TAB-0373CL-S(P)) | ELISA, FuncS, Inhib | Human scFv |
TAB-0373CL-F(E) | Human Anti-IL18 Recombinant Antibody; Fab Fragment (TAB-0373CL-F(E)) | ELISA, FuncS, Inhib | Human Fab |
TAB-0374CL-F(E) | Human Anti-IL18 Recombinant Antibody; Fab Fragment (TAB-0374CL-F(E)) | ELISA, FuncS, Inhib | Human Fab |
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