Rat Anti-IL18 Recombinant Antibody (clone 2C10); scFv Fragment (CAT#: HPAB-0067-YC-S(P))

Figure 1 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC-S(P)) in DB

Dot Blot analysis of HPAB-0067-YC-S(P) was performed by coating with human IL18 protein (His tag).
HPAB-0067-YC-S(P) incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-His tag as a secondary antibody (1: 2000)

Figure 2 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC-S(P)) in WB

Western blot analysis of HPAB-0067-YC-S(P) was performed by loading human IL18 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0067-YC-S(P) and HRP goat anti-His tag as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Figure 3 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC-S(P)) in ELISA

ELISA analysis of HPAB-0067-YC-S(P) was performed by coating with human IL18 protein (His tag). Then blocked with BSA and incubated with HPAB-0067-YC-S(P). The HRP-conjugated goat anti-His tag as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.