Anti-IL3RA immunotoxin (scFv)-DT (CAT#: AGTO-G045D)
This immunotoxin was achieved by conjugating/fusing the Anti-IL3RA scFv to Diphtheria toxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to IL3RA expressing leukemia cells and the toxin enters cells by receptor-mediated endocytosis. Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme, -II binding and elongation factor into the eukaryotic cell, the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs, thereby preventing protein synthesis and causes cell death. It was designed for treatment of acute Myeloid leukemia.
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Normal Tissue
RNA Expression
Figure 1 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/ihc_selected.jpg
(Immunohistochemical staining of human small intestine shows cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/ihc_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_B_8_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_9_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_8_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_5_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_5_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_9_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (HDLM-2, NB-4, RPMI-8226)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185291-IL3RA
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185291-IL3RA
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Molecule Class
- scFv-DT
- Toxin Description
- Diphtheria toxin (Diphtheriatoxin, DT) is a cell toxin produced by diphtheria, is a protein toxin most commonly used in the construction of recombinant immunotoxin. Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme, -II binding and elongation factor into the eukaryotic cell, the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs, thereby preventing protein synthesis and causes cell death.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Acute Myeloid Leukemia
Target
- Alternative Names
- IL3RA; interleukin 3 receptor, alpha (low affinity); IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra; interleukin-3 receptor subunit alpha; IL-3RA; IL-3R-alpha; CD123 antigen; IL-3R subunit alpha; IL-3 receptor subunit alpha; IL-3 receptor alpha SP2 isoform; Diphtheria toxin; Diphtheria-toxin; DT; DT388; DT389; DT390; DAB425
- Gene ID
- 3563
- UniProt ID
- P26951
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G044E | IL-3-PE immunotoxin | Cytotoxicity assay, Function study | |
| AGTO-G044D | IL-3-DT immunotoxin | Cytotoxicity assay, Function study | |
| AGTO-G045E | Anti-IL3RA immunotoxin (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-L009E | IL3-PE immunotoxin | Cytotoxicity assay, Functional assay | |
| AGTO-L009D | IL3-DT immunotoxin | Cytotoxicity assay, Functional assay |
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