Anti-Human IL4R Recombinant Antibody (12B5) (CAT#: TAB-0004CL)
It is a recombinant antibody targeting the Interleukin-4 receptor subunit alpha (IL4RA), derived from human. It inhibits the binding of human IL-4 to human IL-4 receptor. This antibody has the ability to function as both an IL-4 antagonist and an IL-13 antagonist.
Specific Inquiry
WB
Figure 1 Anti-Human IL4R Recombinant Antibody (TAB-0004CL) in WB
Western blot analysis was performed with 0.1 µg (lane 1), 0.3 µg(lane 2), and 0.6 µg (lane 3) recombinant human IL4R protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with TAB-0004CL at a dilution of 1:500 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:2,000 for one hour. Chemiluminescent was detected.
DB
Figure 2 Anti-Human IL4R Recombinant Antibody (TAB-0004CL) in Dot Blot
Dot Blot analysis of TAB-0004CL was performed by coating with human IL4R protein antigen.
TAB-0004CL incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
ELISA
Figure 3 Anti-Human IL4R Recombinant Antibody (TAB-0004CL) in ELISA
ELISA analysis of TAB-0004CL was performed by coating with recombinant human IL4R antigen. Then blocked with BSA, and incubated with TAB-0004CL. The HRP-conjugated goat anti-human IgG was used as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- Human antibody
- Specificity
- Human
- Clone
- 12B5
Target
- Alternative Names
- IL4R; interleukin 4 receptor; CD124; IL4RA; IL-4RA; interleukin-4 receptor subunit alpha; IL4R nirs variant 1; IL-4 receptor subunit alpha; interleukin-4 receptor alpha chain
- Gene ID
- 3566
- UniProt ID
- P24394
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L079E | IL4-PE immunotoxin | Cytotoxicity assay, Functional assay | |
| AGTO-L079D | IL4-DT immunotoxin | Cytotoxicity assay, Functional assay |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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