Anti-Human IL4R Recombinant Antibody (12B5) (CAT#: TAB-0004CL)

Figure 1 Anti-Human IL4R Recombinant Antibody (TAB-0004CL) in WB

Western blot analysis was performed with 0.1 µg (lane 1), 0.3 µg(lane 2), and 0.6 µg (lane 3) recombinant human IL4R protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with TAB-0004CL at a dilution of 1:500 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:2,000 for one hour. Chemiluminescent was detected.

Figure 2 Anti-Human IL4R Recombinant Antibody (TAB-0004CL) in Dot Blot

Dot Blot analysis of TAB-0004CL was performed by coating with human IL4R protein antigen.
TAB-0004CL incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)

Figure 3 Anti-Human IL4R Recombinant Antibody (TAB-0004CL) in ELISA

ELISA analysis of TAB-0004CL was performed by coating with recombinant human IL4R antigen. Then blocked with BSA, and incubated with TAB-0004CL. The HRP-conjugated goat anti-human IgG was used as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.