Human Anti-IL6 Recombinant Antibody (TAB-0173CL) (CAT#: TAB-0173CL)

This product is a recombinant Human antibody that recognizes IL6. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.


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ELISA

Figure 1 Anti-Human IL6 Recombinant Antibody (TAB-0173CL) in ELISA

Figure 1 Anti-Human IL6 Recombinant Antibody (TAB-0173CL) in ELISA

ELISA analysis of TAB-0173CL was performed by coating with human IL6 protein (His tag). Then blocked with BSA and incubated with TAB-0173CL. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

WB

Figure 2 Anti-Human IL6 Recombinant Antibody (TAB-0173CL) in WB

Figure 2 Anti-Human IL6 Recombinant Antibody (TAB-0173CL) in WB

Western blot analysis of TAB-0173CL was performed by loading human IL6 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-0173CL and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

DB

Figure 3 Anti-Human IL6 Recombinant Antibody (TAB-0173CL) in DB

Figure 3 Anti-Human IL6 Recombinant Antibody (TAB-0173CL) in DB

Dot Blot analysis of TAB-0173CL was performed by coating with human IL6 protein (His tag).
TAB-0173CL incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control


Specifications

  • Host Species
  • Human
  • Derivation
  • Humanized
  • Type
  • Human IgG1
  • Specificity
  • Human IL6
  • Species Reactivity
  • Human, Cynomolgus, African green monkey
  • Applications
  • ELISA, Block, In vivo
  • Related Disease
  • Diabetic macular edema, wet AMD

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • IL6; interleukin 6; HGF; HSF; BSF2; IL-6; IFNB2; interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; interferon, beta 2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "IL6"

Mouse Antibody

CAT Product Name Application Type
TAB-239 Anti-Human IL6 Recombinant Antibody (Elsilimomab) WB, FuncS, IF, Neut, ELISA, FC, IP IgG1
TAB-0022CL Mouse Anti-IL6 Recombinant Antibody (TAB-0022CL) Elisa, In vivo, FuncS, Block Mouse IgG
TAB-0023CL Mouse Anti-IL6 Recombinant Antibody (TAB-0023CL) Elisa, In vivo, FuncS, Block Mouse IgG
TAB-0024CL Mouse Anti-IL6 Recombinant Antibody (TAB-0024CL) Elisa, In vivo, FuncS, Block Mouse IgG
TAB-0025CL Mouse Anti-IL6 Recombinant Antibody (TAB-0025CL) Elisa, In vivo, FuncS, Block Mouse IgG

Humanized Antibody

CAT Product Name Application Type
TAB-253 Anti-Human IL-6 Recombinant Antibody (Olokizumab) WB, ELISA, FC, IP, FuncS, IF, Neut IgG4 - kappa
TAB-0172CL-S(P) Human Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0172CL-S(P)) ELISA, Block, In vivo Human scFv
TAB-0173CL-S(P) Human Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0173CL-S(P)) ELISA, Block, In vivo Human scFv
TAB-678LC-S(P) Anti-human IL6 scFv Fragment (BA399) ELISA, FC Humanized antibody
TAB-678LC-F(E) Anti-human IL6 Fab Fragment (BA399) ELISA, FC Humanized antibody

Chimeric Antibody

CAT Product Name Application Type
TAB-0026CL-S(P) Mouse Anti-IL6 Recombinant Antibody; scFv Fragment (TAB-0026CL-S(P)) Elisa, In vivo, FuncS, Block Mouse scFv
TAB-0026CL-F(E) Human Anti-IL6 Recombinant Antibody; Fab Fragment (TAB-0026CL-F(E)) Elisa, In vivo, FuncS, Block Chimeric (mouse/human) Fab
TAB-670LC-S(P) Anti-human IL6 scFv Fragment (NTS) ELISA Chimeric antibody (mouse/human)
TAB-670LC-F(E) Anti-human IL6 Fab Fragment (NTS) ELISA Chimeric antibody (mouse/human)
VS3-WK1519 Human Anti-IL6 Recombinant Antibody (clone CLLB8) ELISA, FC Chimeric (mouse/human) IgG1

Fab Glycosylation

CAT Product Name Application Type
Gly-037LC Recombinant Anti-Human IL6 Antibody (Fab glycosylation) ELISA Humanized antibody

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-197LC Recombinant Anti-Human IL6 Antibody (Non-glycosylated) ELISA Humanized antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0284MZ Chicken Anti-Interleukin-6 Polyclonal IgY WB, Indirect ELISA Chicken antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-1474CQ Rat Anti-Il6 Recombinant Antibody (clone 32C11) Block, ELISA, WB Rat IgG2a

MHC Tetramer for Cancer

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-239 Afuco™ Anti-IL6 ADCC Recombinant Antibody (Elsilimomab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC ADCC enhanced antibody
AFC-TAB-097 Afuco™ Anti-IL6 ADCC Recombinant Antibody (Sirukumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody
AFC-TAB-H26 Afuco™ Anti-IL6 ADCC Recombinant Antibody (Enlimomab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-253 Afuco™ Anti-IL6 ADCC Recombinant Antibody (Olokizumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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