Anti-human ITGAE Recombinant Antibody (3G6) (TAB-742LC) (CAT#: TAB-742LC)

Figure 1 Fluorescent histograms showing binding of mAb 3G6 (IgG1) to transfected K562 cells that expressed an αEβ7 integrin under a variety of buffer conditions.

The transfected cells were stained with isotype control antibody (human IgG1) in standard staining buffer (PBS/5% FBS) (FIG. 1A), with mAb 3G6 (IgG1) in standard staining buffer (FIG. 1B) or in buffer that contained EDTA (5 mM; FIG. 1C), in buffer that contained MnCl2 (1 mM; FIG. 1D), in buffer that contained MgCl2 (1 mM; FIG. 1E), in buffer that contained CaCl2 (1 mM; FIG. 1F), in buffer that contained MgCl2 and CaCl2 (1 mM each; FIG. 1G) or in buffer that contained MgCl2, CaCl2 and MnCl2 (1 mM each; FIG. 1H), and bound antibody was detected using a fluorescein isothiocyanate (FITC) labeled anti-human IgG antibody. The results show that binding of mAb 3G6 (IgG1) to the transfected cells was enhanced in buffer that contained Mn2+ (FIGS. 1D and 1H) and inhibited in buffer that contained EDTA (FIG. 1C) relative to binding in standard buffer.