Recombinant Human Antibody (AL-57) is capable of binding to ITGAL, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-ITGAL mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-ITGAL proteins mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. AL-57 functions as an ICAM-1 mimetic antibody that exhibits several key features of the ICAM-1/LFA-1 interaction. Not only do AL-57 and ICAM-1 both bind progressively better as LFA-1 affinity increases, they both require Mg2+ for binding.
Figure 1 SPR analysis of binding by the αL I domains to Abs AL-57 and MHM24.
The HA (K287C/K294C), IA (L161C/F299C), or low-affinity WT I domain was perfused onto immobilized Abs in the presence of 1 mM MgCl2. The concentration series for MHM24 was 3.91, 7.81, 15.63, 31.25, 62.5, 125, 250, and 500 nM. For AL-57, the concentration series was 15.6, 31.3, 62.5, 125, and 250 nM for the HA I domain and 31.25, 62.5, 125, 250, and 500 nM for WT and IA I domains. In all cases, higher concentrations gave higher responses (except that differences are not visible for WT with AL-57 in Mg2+).
Shimaoka, M., Kim, M., Cohen, E. H., Yang, W., Astrof, N., Peer, D., ... & Springer, T. A. (2006). AL-57, a ligand-mimetic antibody to integrin LFA-1, reveals chemokine-induced affinity up-regulation in lymphocytes. Proceedings of the National Academy of Sciences, 103(38), 13991-13996.
Figure 2 SPR analysis of binding by the αL I domains to Abs AL-57 and MHM24.
The HA (K287C/K294C), IA (L161C/F299C), or low-affinity WT I domain was perfused onto immobilized Abs in the presence of 10 mM EDTA. The concentration series for MHM24 was 3.91, 7.81, 15.63, 31.25, 62.5, 125, 250, and 500 nM. For AL-57, the concentration series was 15.6, 31.3, 62.5, 125, and 250 nM for the HA I domain and 31.25, 62.5, 125, 250, and 500 nM for WT and IA I domains. In all cases, higher concentrations gave higher responses (except that differences are not visible for HA with AL-57 in EDTA).
Shimaoka, M., Kim, M., Cohen, E. H., Yang, W., Astrof, N., Peer, D., ... & Springer, T. A. (2006). AL-57, a ligand-mimetic antibody to integrin LFA-1, reveals chemokine-induced affinity up-regulation in lymphocytes. Proceedings of the National Academy of Sciences, 103(38), 13991-13996.
Figure 3 Acidic residue D101 of AL-57 is critical for binding.
Binding of parental and mutant AL-57 IgG (3 μg/ml) to the isolated, locked HA I domain expressed on K562 cells was examined by immunofluorescent flow cytometry in the presence of 1 mM Mg2+ (filled histograms) or 5 mM EDTA (open histograms). Binding of AL-57D101A in Mg2+ and EDTA is hardly distinguishable from background binding of control IgG.
Shimaoka, M., Kim, M., Cohen, E. H., Yang, W., Astrof, N., Peer, D., ... & Springer, T. A. (2006). AL-57, a ligand-mimetic antibody to integrin LFA-1, reveals chemokine-induced affinity up-regulation in lymphocytes. Proceedings of the National Academy of Sciences, 103(38), 13991-13996.
Figure 4 Binding of AL-57 IgG to WT LFA-1 activated by agonists as determined by immunofluorescent flow cytometry.
K562 transfectants expressing WT LFA-1 and IL-15-cultured human primary T lymphocytes were stained for 20 min at 37°C with 20 μg/ml AL-57, TS2/4, or isotype-matched human or mouse control IgG in Hepes-buffered saline containing 1 mM MgCl2/1 mM CaCl2, 5 mM MgCl2/1 mM EGTA, or 1 mM MnCl2. Cells were washed and stained with FITC-conjugated goat anti-human or anti-mouse Abs. Staining with AL-57 and TS2/4 is shown as solid lines, and background staining with control IgGs is shown as dashed lines. Numbers within the panels show the specific mean fluorescence intensity of AL-57 and TS2/4.
Shimaoka, M., Kim, M., Cohen, E. H., Yang, W., Astrof, N., Peer, D., ... & Springer, T. A. (2006). AL-57, a ligand-mimetic antibody to integrin LFA-1, reveals chemokine-induced affinity up-regulation in lymphocytes. Proceedings of the National Academy of Sciences, 103(38), 13991-13996.
Figure 5 The kinetics of LFA-1 activation after stimulation with CXCL-12.
Representative histograms for a time course of AL-57 epitope expression are shown. T lymphocytes were stimulated with 50 ng/ml CXCL-12 for the indicated time period. Cells were stained with Fab AL-57 or control Fab labeled with Alexa 488 for 2 min by adding Abs 2 min before the end of stimulation. Cells were then fixed and subjected to flow cytometry. Background staining with control Fab is shown in the open histograms. Numbers within the panels show specific mean fluorescence intensity values for Fab AL-57. At 0, 10, and 20 min, differences between AL-57 Fab and control Fab binding are hardly visible.
Shimaoka, M., Kim, M., Cohen, E. H., Yang, W., Astrof, N., Peer, D., ... & Springer, T. A. (2006). AL-57, a ligand-mimetic antibody to integrin LFA-1, reveals chemokine-induced affinity up-regulation in lymphocytes. Proceedings of the National Academy of Sciences, 103(38), 13991-13996.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-024 | Anti-Human CD11A Recombinant Antibody (Efalizumab) | Neut, ELISA, IF, IP, FuncS, FC, WB | IgG1 - kappa |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-108 | Anti-Human CD11a Recombinant Antibody (Odulimomab) | Neut, ELISA, IF, IP, FuncS, FC, ICC | IgG1 |
TAB-459CL | Mouse Anti-ITGAL Recombinant Antibody (TAB-459CL) | ELISA | Mouse IgG |
TAB-1579CL | Rat Anti-ITGAL Recombinant Antibody (TAB-1579CL) | Block | Rat IgG2a, κ |
TAB-1579CL-S(P) | Rat Anti-ITGAL Recombinant Antibody; scFv Fragment (TAB-1579CL-S(P)) | Block | Rat scFv |
TAB-1579CL-F(E) | Rat Anti-ITGAL Recombinant Antibody; Fab Fragment (TAB-1579CL-F(E)) | Block | Rat Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBW-159 | Human Anti-ITGAL Recombinant Antibody (clone AL-57); Fab Fragment | WB | Human Fab |
NS-018CN-F(E) | Human Anti-ITGAL Recombinant Antibody (clone MHM24); Fab Fragment | FC, ELISA | Human Fab |
HPAB-0487-WJ-F(E) | Mouse Anti-ITGAL Recombinant Antibody (clone S6F1); Fab Fragment | ELISA | Mouse Fab |
HPAB-S0088-YC-F(E) | Human Anti-ITGAL Recombinant Antibody (clone chMHM24); Fab Fragment | Inhib | Chimeric (mouse/human) Fab |
HPAB-S0089-YC-F(E) | Human Anti-ITGAL Recombinant Antibody (clone huMHM24); Fab Fragment | Inhib | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBW-159 | Human Anti-ITGAL Recombinant Antibody (clone AL-57); scFv Fragment | WB | Human scFv |
NS-018CN-S(P) | Human Anti-ITGAL Recombinant Antibody (clone MHM24); scFv Fragment | FC, ELISA | Human scFv |
HPAB-0487-WJ-S(P) | Mouse Anti-ITGAL Recombinant Antibody (clone S6F1); scFv Fragment | ELISA | Mouse scFv |
HPAB-S0088-YC-S(P) | Human Anti-ITGAL Recombinant Antibody (clone chMHM24); scFv Fragment | Inhib | Mouse scFv |
HPAB-S0089-YC-S(P) | Human Anti-ITGAL Recombinant Antibody (clone huMHM24); scFv Fragment | Inhib | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-755LC | Anti-human ITGAL Recombinant Antibody (Chimeric S6F1) (TAB-755LC) | ELISA | Chimeric antibody (mouse/human) |
TAB-755LC-S(P) | Anti-human ITGAL scFv Fragment (Chimeric S6F1) | ELISA | Chimeric antibody (mouse/human) |
TAB-755LC-F(E) | Anti-human ITGAL Fab Fragment (Chimeric S6F1) | ELISA | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1559CQ | Recombinant Mouse Anti-ITGAL Antibody (38) | FC, ICC/IF, IHC-Fr, IP, Neut | IgG2a |
NEUT-1563CQ | Recombinant Mouse Anti-ITGAL Antibody (CBL227) | FC, IHC, IP, Neut | IgG2a |
NEUT-1565CQ | Recombinant Rat Anti-Itgal Antibody (121/7) | FC, IP, Neut, WB | IgG2a |
NEUT-1568CQ | Recombinant Rat Anti-Itgal Antibody (I21/7) | Neut, FC, IHC, IHC-Fr, IP, ICC | IgG2a, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1560CQ | Recombinant Mouse Anti-ITGAL Antibody (HI111) | FC, BL | IgG1, κ |
NEUT-1561CQ | Recombinant Mouse Anti-ITGAL Antibody (MEM-83) | BL, FC, FuncS, IP | IgG1 |
NEUT-1562CQ | Recombinant Mouse Anti-ITGAL Antibody (TS-1/22.1.1.13) | BL, FC | IgG1 |
NEUT-1564CQ | Recombinant Mouse Anti-ITGAL Antibody (R7-1) | BL, FC | IgG1 |
NEUT-1566CQ | Recombinant Rat Anti-Itgal Antibody (M17/4) | IP, IHC-Fr, FuncS, FC, BL | IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1878 | Hi-Affi™ Recombinant Rabbit Anti-ITGAL Monoclonal Antibody (DS1878AB) | BL, FC, ICC/IF, IHC-P, IP, WB | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NS-018CN | Human Anti-ITGAL Recombinant Antibody (clone MHM24) | FC, ELISA, Inhib, FuncS | Human IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-0267-CN | Recombinant Rabbit Anti-ITGAL Monoclonal Antibody (CBACN-104) | WB, IF, IHC, IP, FC | Rabbit IgG |
FAMAB-1185CQ | Mouse Anti-ITGAL Recombinant Antibody (clone YTH 81.5) | FC | Mouse IgG1 |
ZG-1058F | Mouse Anti-ITGAL Recombinant Antibody (clone NKI (SPV)-L1) | FC, IP, ICC | Mouse IgG1 |
ZG-1059F | Mouse Anti-ITGAL Recombinant Antibody (clone NKI(SPV)-L16) | FC, IP | Mouse IgG2a |
ZG-1061F | Mouse Anti-ITGAL Recombinant Antibody (clone NKI(SPV)-L7) | FC, IP | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-108 | Afuco™ Anti-ITGAL ADCC Recombinant Antibody (Odulimomab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
AFC-TAB-024 | Afuco™ Anti-ITGAL ADCC Recombinant Antibody (Efalizumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0066SW | Recombinant Llama Anti-ITGAL Single Domain Antibody (138-B10) | ELISA, WB | Llama VHH |
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