Human Anti-ITGAL Recombinant Antibody (clone MHM24) (CAT#: NS-018CN)

Figure 1 Inhibition of human Jurkat T-cells to normal human keratinocytes by murine MHM24 (filled circles), chimeric MHM24 (open triangles), humanized MHM24 (HuIG1) (filled squares), and a human IgG1 isotype control (+).

Percent binding measured by fluorescence of labeled Jurkat cells.

Figure 2 Human mixed lymphocyte response assy (MLR) is blocked by murine MHM24 (filled circles), humanized MHM24 (HuIgG1) (filled squares), and a humanized isotype IgG1 control (filled diamond).

Percent stimulation index (%SI) is the ratio of the response at a given MAb concentration to the maximal response with no MAb present. Data is representative of multiple assays using at least two different stimulator/responder pairs.

Figure 3 Inhibition of binding of rhesus lymphocytes to normal human keratinocytes (FIG. A), rhesus lymphocytes to recombinant human ICAM-1 coated on plates (FIG. B), and rhesus/human CD11a chimera-transfected 293 cells to normal human keratinocytes (FIG. C).

Inhibition by rhesus-binding MHM24 (RhIgG1) (filled squares), anti-CD18 MHM23 (filled circles), a human IgG1 isotype control (+) (FIGS. A and C), and a murine IgG1 isotype control (+) (FIG. B). Percent binding measured by fluorescence of labeled lymphocytes (FIGS. A and B) or labeled 293 cells (FIG. C).