Recombinant Mouse Anti-ITGB3 Antibody (25E11)
CAT#: NEUT-1598CQ
This antibody is a mouse monoclonal antibody that binds specifically to CD61, and it can neutralize the bioactivity of CD61.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27852/281_B9_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27852/ihc_selected.jpg
Normal Tissue
Figure 3 Bone marrow
Hematopoietic cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27852/64673_B_4_4.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (EFO-21, HEL, RPTEC TERT1, U-87 MG, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000259207-ITGB3
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Specifications
- Immunogen
- Normal blood mononuclear cells activated by mixed lymphocyte reaction
- Host Species
- Mouse
- Type
- IgM, κ
- Specificity
- Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain)
- Species Reactivity
- Human
- Clone
- 25E11
- Applications
- Used for immunoassay techniques such as: Flow Cytometry; Immunofluorescence; Inhibition
- Conjugate
- Unconjugated
Product Property
- Purification
- PEG precipitation
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze-thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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