What is the specific format of this antibody, and how does it differ from a whole IgG?

This product is a di-Fab fragment based on the Alacizumab clone. A di-Fab consists of two Fab fragments linked together, lacking the Fc region found in a whole IgG. This format retains bivalency (two binding sites) but has a smaller molecular size and eliminates Fc-mediated effector functions, which can be beneficial for certain receptor-binding or internalization studies where Fc interaction is undesirable.

Does this antibody bind to human VEGFR2, and is it suitable for blocking studies?

Yes, this antibody targets KDR, also known as VEGFR2 (Vascular Endothelial Growth Factor Receptor 2). The Alacizumab clone is known for its ability to bind this receptor. Being a specific binder, it is suitable for research applications investigating receptor occupancy, ligand blocking, and the downstream signaling pathways of VEGF in endothelial cells.

Humanized Anti-KDR Recombinant Antibody (clone Alacizumab); di - Fab' Fragment

CAT#: TAB-202

This product is a humanized (from mouse) di - Fab' antibody that can recognize human KDR.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HEL

Immunofluorescent staining of human cell line HEL shows localization to nuclear membrane & vesicles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73577/1825_D8_31_cr5ac340707076b_selected.jpg

Normal Tissue Figure 2 Placenta

High expression

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4028/11467_A_2_7_val_selected.jpg

Normal Tissue Figure 3 Colon

Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4028/11467_A_8_3.jpg

Normal Tissue Figure 4 Kidney

Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4028/11467_A_7_5.jpg

Normal Tissue Figure 5 Testis

Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4028/11467_A_6_6.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (HUVEC TERT2, TIME)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128052-KDR

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Specifications

Immunogen
Recombinant human soluble extracellular KDR (110 kDa) native protein.

Host Species
Human

Derivation
Humanized (from mouse)

Type
Humanized (from mouse) di - Fab'

Specificity
Human KDR

Species Reactivity
Human

Clone
Alacizumab

Applications
IF, IP, Neut, FuncS, ELISA, FC, ICC

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in Immunofluorescence, Immunoprecipitation, Neutralization, Functional Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry.

Target

Alternative Names
FLK1; CD309; VEGFR; VEGFR2; vascular endothelial growth factor receptor 2; fetal liver kinase-1; kinase insert domain receptor (a type III receptor tyrosine kinase); protein-tyrosine kinase receptor Flk-1; soluble VEGFR2; tyrosine kinase growth factor receptor

Gene ID
3791

UniProt ID
P35968

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

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Breast cancer biomarkers at key points during disease progression

High Affinity Fragment

The di-Fab format of the Alacizumab clone offers a unique advantage for our VEGFR2 (KDR) internalization studies. The smaller size compared to full-length IgG allows for different binding kinetics which we found useful. We used it to track receptor turnover in endothelial cells. The antibody fragment was stable and retained high binding affinity, providing clear fluorescent signals in our microscopy experiments.

Effective for Signaling Research

We employed this Anti-KDR di-Fab to investigate the blocking of VEGF signaling pathways. It effectively bound to the receptor and we could measure the downstream effects on phosphorylation. The recombinant production ensures that the product is free of animal-derived contaminants, which is important for our serum-free cell culture conditions. A very specialized and effective tool for angiogenesis research.

Q&As

What is the specific format of this antibody, and how does it differ from a whole IgG?

A: This product is a di-Fab fragment based on the Alacizumab clone. A di-Fab consists of two Fab fragments linked together, lacking the Fc region found in a whole IgG. This format retains bivalency (two binding sites) but has a smaller molecular size and eliminates Fc-mediated effector functions, which can be beneficial for certain receptor-binding or internalization studies where Fc interaction is undesirable.
Does this antibody bind to human VEGFR2, and is it suitable for blocking studies?

A: Yes, this antibody targets KDR, also known as VEGFR2 (Vascular Endothelial Growth Factor Receptor 2). The Alacizumab clone is known for its ability to bind this receptor. Being a specific binder, it is suitable for research applications investigating receptor occupancy, ligand blocking, and the downstream signaling pathways of VEGF in endothelial cells.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-202.pdf

MSDS

MSDS - TAB-202.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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