Human Anti-LCAT Recombinant Antibody (clone Fab1)
CAT#: PABL-631
Recombinant Human Antibody (Fab1) is capable of binding to LCAT, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-LCAT mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-LCAT proteins mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Published Data
Gene Expression
Figure 1 Fab1 inhibits LCAT activity on lipid-associated but not soluble substrates.
A: Increasing concentrations of mAb1 (the parent antibody of Fab1) inhibit the full LCAT reaction and prevent the formation of CEs on ApoA-I-containing proteoliposome particles. Activity shown is per 0.05 μg of LCAT enzyme. Similar inhibition was seen with Fab1 (data not shown). B: LCAT and LCAT/Fab1 complex have similar phospholipase activity on soluble substrates.
Piper, D. E., Romanow, W. G., Gunawardane, R. N., Fordstrom, P., Masterman, S., Pan, O., ... & Wang, Z. (2015). The high-resolution crystal structure of human LCAT. Journal of lipid research, 56(9), 1711-1719.
Figure 2 Crystal structure of LCAT.
A: The overall structure of the LCAT/Fab1 complex. LCAT is shown in a rainbow multicolor depiction from the N to C terminus (blue to red). Fab1 (gray) binds to subdomain 1 at the N-terminal region of LCAT. B: Diagram of LCAT showing the secondary structure and chain trace of the protein. Secondary structure elements within the α/β hydrolase domain are numbered in canonical form. Secondary structure elements outside of the α/β hydrolase domain are labeled sequentially. The α/β hydrolase domain is colored blue, subdomain 1 is colored violet, and subdomain 2 is colored green. C: Structure of LCAT only with Fab1 removed for clarity. Regions of LCAT are colored as in B. The catalytic triad residues, N-linked carbohydrate sites, and disulfide bonds of LCAT are highlighted in stick representation. D: 2Fo-Fc electron density contoured at 1 σ focusing on the catalytic triad and β sheet of the α/β hydrolase core. Amino acids of the catalytic triad are labeled. Strong electron density for the Zn ion is present next to His377.
Piper, D. E., Romanow, W. G., Gunawardane, R. N., Fordstrom, P., Masterman, S., Pan, O., ... & Wang, Z. (2015). The high-resolution crystal structure of human LCAT. Journal of lipid research, 56(9), 1711-1719.
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Normal Tissue
Figure 1 IHC staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44767/if_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (BEWO, Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000213398-LCAT
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Specifications
- Immunogen
- Human Lecithin-Cholesterol Acyltransferase
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Specificity
- Tested positive against LCAT
- Species Reactivity
- Human
- Clone
- Fab1
- Applications
- WB, IF, FuncS
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1813z | Mouse Anti-LCAT Recombinant Antibody (clone 36G2) | WB, ELISA, IF, IP | Mouse IgG1, κ |
| PABL-258 | Human Anti-LCAT Recombinant Antibody (PABL-258) | WB, ELISA, FuncS | Human IgG |
| HPAB-0899-WJ | Human Anti-LCAT Recombinant Antibody (HPAB-0899-WJ) | ELISA | Humanized IgG |
| HPAB-0900-WJ | Human Anti-LCAT Recombinant Antibody (HPAB-0900-WJ) | ELISA | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-258 | Human Anti-LCAT Recombinant Antibody; scFv Fragment (PSBL-258) | WB, ELISA, FuncS | Human scFv |
| PSBL-626 | Human Anti-LCAT Recombinant Antibody (clone Fab1); scFv Fragment | WB, IF, FuncS | Human scFv |
| HPAB-0899-WJ-S(P) | Human Anti-LCAT Recombinant Antibody; scFv Fragment (HPAB-0899-WJ-S(P)) | ELISA | Humanized scFv |
| HPAB-0900-WJ-S(P) | Human Anti-LCAT Recombinant Antibody; scFv Fragment (HPAB-0900-WJ-S(P)) | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-258 | Human Anti-LCAT Recombinant Antibody; Fab Fragment (PFBL-258) | WB, ELISA, FuncS | Human Fab |
| PFBL-626 | Human Anti-LCAT Recombinant Antibody (clone Fab1); Fab Fragment | WB, IF, FuncS | Human Fab |
| HPAB-0899-WJ-F(E) | Human Anti-LCAT Recombinant Antibody; Fab Fragment (HPAB-0899-WJ-F(E)) | ELISA | Humanized Fab |
| HPAB-0900-WJ-F(E) | Human Anti-LCAT Recombinant Antibody; Fab Fragment (HPAB-0900-WJ-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0647CL | Human Anti-LCAT Recombinant Antibody (TAB-0647CL) | FuncS | Human IgG |
| TAB-0647CL-S(P) | Human Anti-LCAT Recombinant Antibody; scFv Fragment (TAB-0647CL-S(P)) | ELISA | Human scFv |
| TAB-0647CL-F(E) | Human Anti-LCAT Recombinant Antibody; Fab Fragment (TAB-0647CL-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2022 | Hi-Affi™ Recombinant Rabbit Anti-LCAT Monoclonal Antibody (DS2022AB) | FC, ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY61 | Human Anti-LCAT Agonistic Antibody (VS-0724-XY61) | ELISA, SPR, Agonistic assays | Human IgG2, lambda |
| VS-0724-XY62 | Human Anti-LCAT Agonistic Antibody (clone 18E5) | ELISA, SPR, Agonistic assays | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3958 | Anti-LCAT Immunohistochemistry Kit | IHC |
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