Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0103-CN-S(P)) (CAT#: HPAB-0103-CN-S(P))
This product is a recombinant mouse antibody scFv fragment that recognizes LGR5. The antibody was purified by affinity chromatography.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/1404_H2_1_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/1404_H2_1_selected.jpg
Figure 2 IHC staining of human colon
(Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/ihc_selected.jpg
(Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/103_B2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/103_B2_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/102_B2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/102_B2_1_blue_red_green.jpg
Figure 5 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_B_7_5.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_B_7_5.jpg
Figure 6 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_3.jpg
Figure 7 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_7_4.jpg
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_7_4.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_5.jpg
Figure 9 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_6_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_6_6.jpg
Figure 10 RNA cell line category: Group enriched (Hep G2, SH-SY5Y)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139292-LGR5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139292-LGR5
Specifications
- Immunogen
- Human LgR5 extracellular domain (ECD; amino acids 22-557) with a C-terminal His-tagged Fc
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse scFv
- Species Reactivity
- Human, Mouse
- Applications
- WB, ELISA, FC, IHC
- Related Disease
- LgR5-associated conditions, such as cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1650-VHH | Recombinant Anti-Human LGR5 VHH Single Domain Antibody | WB, ICC, ChiP, FA, ELISA | Llama VHH |
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