Mouse Anti-LOXL2 Recombinant Antibody (HPAB-0160-LSX)
CAT#: HPAB-0160-LSX
This product is a recombinant mouse antibody that recognizes LOXL2. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36257/847_B2_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56542/985_A11_1_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows moderate positivity in exocrine cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25848/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/36257/752_B2_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25848/56663_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25848/56663_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25848/56663_A_8_4.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25848/56663_A_4_6.jpg
Normal Tissue
Figure 9 Placenta
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/25848/56663_A_3_7.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+ SV40 Large T+ RasG12V, fHDF/TERT166, TIME, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000134013-LOXL2
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Specifications
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Human LOXL2
- Species Reactivity
- Human
- Applications
- ELISA
- Related Disease
- Fibrotic condition
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
REVIEWS AND Q&AS
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Citations
-
Fan, Zhiyong, et al. "LOXL2 upregulates hypoxia-inducible factor-1α signaling through Snail-FBP1 axis in hepatocellular carcinoma cells." Oncology reports 43.5 (2020): 1641-1649. https://doi.org/10.3892/or.2020.7541This research investigates the role of Lysyl oxidase-like 2 (LOXL2) in hepatocellular carcinoma (HCC) progression, focusing on its regulatory effect on hypoxia-inducible factor-1α (HIF-1α) signaling. The study demonstrates that LOXL2 inhibits the expression of fructose-1,6-biphosphatase (FBP1) in a Snail-dependent manner, which subsequently enhances glycolysis in HCC cell lines. Importantly, the research reveals that intracellular LOXL2 activity, rather than extracellular LOXL2, is responsible for upregulating HIF-1α/VEGF signaling pathways through the Snail-FBP1 axis. This effect was abolished when using LOXL2 inhibitors, suggesting therapeutic potential.
Creative Biolabs provided an anti-human LOXL2 therapeutic antibody (Simtuzumab; CAT#: HPAB-0160-LSX) that was used to evaluate the binding between the antibody and extracellular LOXL2 protein in immunoprecipitation assays. This antibody helped researchers distinguish between the roles of intracellular and extracellular LOXL2, leading to the critical finding that intracellular LOXL2 activity is primarily responsible for Snail upregulation and subsequent metabolic changes in HCC cells, which represents a significant advancement in understanding potential therapeutic targets for hepatocellular carcinoma.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "LOXL2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1260z | Mouse Anti-LOXL2 Recombinant Antibody (clone 19H6) | WB, IP, IF, ELISA | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-890 | Anti-Human LOXL2 Recombinant Antibody (Simtuzumab) | Inhib, Agonist | IgG4 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0412ZL | Rabbit anti-Human LOXL2 Antibody | ICC, IF, FACS, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0693CL | Mouse Anti-LOXL2 Recombinant Antibody (TAB-0693CL) | IF, ELISA, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0693CL-S(P) | Mouse Anti-LOXL2 Recombinant Antibody; scFv Fragment (TAB-0693CL-S(P)) | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0693CL-F(E) | Mouse Anti-LOXL2 Recombinant Antibody; Fab Fragment (TAB-0693CL-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2075 | Hi-Affi™ Recombinant Rabbit Anti-LOXL2 Monoclonal Antibody (DS2075AB) | ICC, IF, FC, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0161-LSX | Human Anti-LOXL2 Recombinant Antibody (HPAB-0161-LSX) | ELISA, WB, RIA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0162-LSX | Mouse Anti-LOXL2 Recombinant Antibody (HPAB-0162-LSX) | ELISA, WB, RIA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0160-LSX-S(P) | Mouse Anti-LOXL2 Recombinant Antibody; scFv Fragment (HPAB-0160-LSX-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0161-LSX-S(P) | Human Anti-LOXL2 Recombinant Antibody; scFv Fragment (HPAB-0161-LSX-S(P)) | ELISA, WB, RIA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0162-LSX-S(P) | Mouse Anti-LOXL2 Recombinant Antibody; scFv Fragment (HPAB-0162-LSX-S(P)) | ELISA, WB, RIA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0160-LSX-F(E) | Mouse Anti-LOXL2 Recombinant Antibody; Fab Fragment (HPAB-0160-LSX-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0161-LSX-F(E) | Human Anti-LOXL2 Recombinant Antibody; Fab Fragment (HPAB-0161-LSX-F(E)) | ELISA, WB, RIA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0162-LSX-F(E) | Mouse Anti-LOXL2 Recombinant Antibody; Fab Fragment (HPAB-0162-LSX-F(E)) | ELISA, WB, RIA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0163-LSX | Mouse Anti-LOXL2 Recombinant Antibody (HPAB-0163-LSX) | ELISA, WB, RIA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0163-LSX-S(P) | Mouse Anti-LOXL2 Recombinant Antibody; scFv Fragment (HPAB-0163-LSX-S(P)) | ELISA, WB, RIA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0163-LSX-F(E) | Mouse Anti-LOXL2 Recombinant Antibody; Fab Fragment (HPAB-0163-LSX-F(E)) | ELISA, WB, RIA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-890 | Afuco™ Anti-LOXL2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-890) | FC, IP, ELISA, Neut, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1256 | Anti-LOXL2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY144 | Human Anti-LOXL2 (clone Simtuzumab) scFv-Fc Chimera | Inhib | Human IgG4, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4072 | Anti-Mouse LOXL2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4071 | Anti-Human LOXL2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4073 | Anti-Rat LOXL2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC209 | Recombinant Anti-LOXL2 Eliminating Antibody, pH-Sensitive (VS-0625-YC209) | Antigen-Sweeping In Vivo. | Human IgG4 kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC240 | SmartAb™ Recombinant Anti-LOXL2 pH-dependent Antibody (VS-0825-YC240) | ELISA | Mouse IgG |
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