Mouse Anti-LRP6 Recombinant Antibody (clone mAb135) (CAT#: PABC-205)

Recombinant Mouse Antibody (mAb135) is capable of binding to LRP6, Chinese Hamster Ovary cells (CHO). This antibody enhanced Wnt dependent TCF reporter activation and antagonized DKK1 dependent inhibition of Wnt3A signaling, suggesting a role in modulation of LRP6 function. It completely protected LRP6 from DKK1 dependent internalization.


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  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
FC

Figure 1 mAb135 recognizes endogenous LRP6 with high-affinity.

Figure 1 mAb135 recognizes endogenous LRP6 with high-affinity.

293T cells were transfected with indicated HA-tagged LRP6 constructs. Transfected cells were stained with mAb135 antibodies and analyzed by flow cytometry.

Binnerts, M. E., Tomasevic, N., Bright, J. M., Leung, J., Ahn, V. E., Kim, K. A., ... & Zhou, M. (2009). The first propeller domain of LRP6 regulates sensitivity to DKK1. Molecular biology of the cell, 20(15), 3552-3560.

WB

Figure 2 mAb135 enhances Wnt signaling.

Figure 2 mAb135 enhances Wnt signaling.

HEK 293 cells were treated with increasing amounts of Wnt3A in the absence or presence of mAb135 (10 μg/ml) and analyzed for cytosolic beta-catenin by Western blotting.

Binnerts, M. E., Tomasevic, N., Bright, J. M., Leung, J., Ahn, V. E., Kim, K. A., ... & Zhou, M. (2009). The first propeller domain of LRP6 regulates sensitivity to DKK1. Molecular biology of the cell, 20(15), 3552-3560.

FuncS

Figure 3 mAb135 antagonizes DKK1.

Figure 3 mAb135 antagonizes DKK1.

HEK293 A6 cells were incubated with increasing amounts of mAb135 IgG before treament with Wnt3A (200 ng/ml) in the presence or absence of DKK1 (400 ng/ml).

Binnerts, M. E., Tomasevic, N., Bright, J. M., Leung, J., Ahn, V. E., Kim, K. A., ... & Zhou, M. (2009). The first propeller domain of LRP6 regulates sensitivity to DKK1. Molecular biology of the cell, 20(15), 3552-3560.

IF

Figure 4 mAb135 inhibits DKK1-dependent internalization of LRP6.

Figure 4 mAb135 inhibits DKK1-dependent internalization of LRP6.

HEK-293 cells were cotransfected with LRP6-HA and Kremen1 constructs, pretreated with mAb135 (5 μg/ml) for 1 h followed by treatment with recombinant DKK1 (200 ng/ml) for 10 min. Distribution of
immunostained HA-tagged LRP6 was evaluated by confocal microscopy.

Binnerts, M. E., Tomasevic, N., Bright, J. M., Leung, J., Ahn, V. E., Kim, K. A., ... & Zhou, M. (2009). The first propeller domain of LRP6 regulates sensitivity to DKK1. Molecular biology of the cell, 20(15), 3552-3560.

Inhib

Figure 5 mAb135 inhibits DKK1-dependent internalization of LRP6.

Figure 5 mAb135 inhibits DKK1-dependent internalization of LRP6.

HEK-293 cells were cotransfected with LRP6-HA and Kremen1 constructs, pretreated with mAb135 (5 μg/ml) for 1 h followed by treatment with recombinant DKK1 (200 ng/ml) for 10 min.

Binnerts, M. E., Tomasevic, N., Bright, J. M., Leung, J., Ahn, V. E., Kim, K. A., ... & Zhou, M. (2009). The first propeller domain of LRP6 regulates sensitivity to DKK1. Molecular biology of the cell, 20(15), 3552-3560.

WB

Figure 6 mAb135 and Dkk1 can both bind to LRP6 (1-4).

Figure 6 mAb135 and Dkk1 can both bind to LRP6 (1-4).

LRP6(1-4)–Dkk1 complex at 2 μM wasincubated with the indicated concentration of mAb135, run on native PAGE, and analyzed by western blot with anti-Dkk1. The antibody shifts the LRP6(1-4)–Dkk1 complex upward to the middle band, which still contains Dkk1 as shown by the western blot.

Ahn, V. E., Chu, M. L. H., Choi, H. J., Tran, D., Abo, A., & Weis, W. I. (2011). Structural basis of Wnt signaling inhibition by Dickkopf binding to LRP5/6. Developmental cell, 21(5), 862-873.


Specifications

  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human LRP6
  • Species Reactivity
  • Human
  • Clone
  • mAb135
  • Applications
  • FC, WB, FuncS, IF, Inhib

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Immunofluorescence, Function Assay, Flow Cytometry, Western Blot and Inhibition.

Target

  • Alternative Names
  • LRP6; low density lipoprotein receptor-related protein 6; ADCAD2; low-density lipoprotein receptor-related protein 6; LRP-6

Related Resources

  • Related Signaling Pathways
  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone mAb135"

See other products for "LRP6"

Mouse Antibody

CAT Product Name Application Type
TAB-0092CL Anti-Human LRP6 Recombinant Antibody (77.2) ELISA, FC, Inhib, FuncS, Internalization
TAB-0093CL Anti-Human LRP6 Recombinant Antibody (135.16) ELISA, FC, Inhib, FuncS, Internalization
TAB-0094CL Anti-Human LRP6 Recombinant Antibody (213.7) ELISA, FC, Inhib, FuncS, Internalization
TAB-0114CL-S(P) Anti-Human LRP6 Recombinant Antibody scFv Fragment (1903.1) ELISA, FC, Inhib, FuncS, Internalization
TAB-0348CL-F(E) Human Anti-LRP6 Recombinant Antibody; Fab Fragment (TAB-0348CL-F(E)) ELISA, FC, Inhib, FuncS, Internalization Chimeric (mouse/human) Fab

Chimeric Antibody

CAT Product Name Application Type
TAB-0115CL Chimeric Anti-Human LRP6 Recombinant Antibody ELISA, FC, Inhib, FuncS, Internalization Chimeric (mouse/human)
TAB-0115CL-S(P) Anti-Human LRP6 Recombinant Antibody scFv Fragment ELISA, FC, Inhib, FuncS, Internalization Chimeric (mouse/human)
TAB-0115CL-F(E) Anti-Human LRP6 Recombinant Antibody Fab Fragment ELISA, FC, Inhib, FuncS, Internalization Chimeric (mouse/human)

Human Antibody

CAT Product Name Application Type
TAB-0705CL Anti-Human LRP6 Recombinant Antibody (Fab004) ELISA, FC, Inhib Human antibody
TAB-0706CL Anti-Human LRP6 Recombinant Antibody (Fab002) ELISA, FC, Inhib Human antibody
TAB-0707CL Human Anti-LRP6 Recombinant Antibody (TAB-0707CL) ELISA, FuncS, Inhib, Antagonist Human IgG
TAB-0708CL Human Anti-LRP6 Recombinant Antibody (TAB-0708CL) WB, Inhib, Block Human IgG
TAB-0705CL-F(E) Anti-Human LRP6 Recombinant Antibody Fab Fragment (Fab004) ELISA, FC Human antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2087 Hi-Affi™ Recombinant Rabbit Anti-LRP6 Monoclonal Antibody (DS2087AB) ICC/IF, WB IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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