Recombinant Mouse Antibody (mAb135) is capable of binding to LRP6, Chinese Hamster Ovary cells (CHO). This antibody enhanced Wnt dependent TCF reporter activation and antagonized DKK1 dependent inhibition of Wnt3A signaling, suggesting a role in modulation of LRP6 function. It completely protected LRP6 from DKK1 dependent internalization.
Figure 1 mAb135 recognizes endogenous LRP6 with high-affinity.
293T cells were transfected with indicated HA-tagged LRP6 constructs. Transfected cells were stained with mAb135 antibodies and analyzed by flow cytometry.
Binnerts, M. E., Tomasevic, N., Bright, J. M., Leung, J., Ahn, V. E., Kim, K. A., ... & Zhou, M. (2009). The first propeller domain of LRP6 regulates sensitivity to DKK1. Molecular biology of the cell, 20(15), 3552-3560.
Figure 2 mAb135 enhances Wnt signaling.
HEK 293 cells were treated with increasing amounts of Wnt3A in the absence or presence of mAb135 (10 μg/ml) and analyzed for cytosolic beta-catenin by Western blotting.
Binnerts, M. E., Tomasevic, N., Bright, J. M., Leung, J., Ahn, V. E., Kim, K. A., ... & Zhou, M. (2009). The first propeller domain of LRP6 regulates sensitivity to DKK1. Molecular biology of the cell, 20(15), 3552-3560.
Figure 3 mAb135 antagonizes DKK1.
HEK293 A6 cells were incubated with increasing amounts of mAb135 IgG before treament with Wnt3A (200 ng/ml) in the presence or absence of DKK1 (400 ng/ml).
Binnerts, M. E., Tomasevic, N., Bright, J. M., Leung, J., Ahn, V. E., Kim, K. A., ... & Zhou, M. (2009). The first propeller domain of LRP6 regulates sensitivity to DKK1. Molecular biology of the cell, 20(15), 3552-3560.
Figure 4 mAb135 inhibits DKK1-dependent internalization of LRP6.
HEK-293 cells were cotransfected with LRP6-HA and Kremen1 constructs, pretreated with mAb135 (5 μg/ml) for 1 h followed by treatment with recombinant DKK1 (200 ng/ml) for 10 min. Distribution of
immunostained HA-tagged LRP6 was evaluated by confocal microscopy.
Binnerts, M. E., Tomasevic, N., Bright, J. M., Leung, J., Ahn, V. E., Kim, K. A., ... & Zhou, M. (2009). The first propeller domain of LRP6 regulates sensitivity to DKK1. Molecular biology of the cell, 20(15), 3552-3560.
Figure 5 mAb135 inhibits DKK1-dependent internalization of LRP6.
HEK-293 cells were cotransfected with LRP6-HA and Kremen1 constructs, pretreated with mAb135 (5 μg/ml) for 1 h followed by treatment with recombinant DKK1 (200 ng/ml) for 10 min.
Binnerts, M. E., Tomasevic, N., Bright, J. M., Leung, J., Ahn, V. E., Kim, K. A., ... & Zhou, M. (2009). The first propeller domain of LRP6 regulates sensitivity to DKK1. Molecular biology of the cell, 20(15), 3552-3560.
Figure 6 mAb135 and Dkk1 can both bind to LRP6 (1-4).
LRP6(1-4)–Dkk1 complex at 2 μM wasincubated with the indicated concentration of mAb135, run on native PAGE, and analyzed by western blot with anti-Dkk1. The antibody shifts the LRP6(1-4)–Dkk1 complex upward to the middle band, which still contains Dkk1 as shown by the western blot.
Ahn, V. E., Chu, M. L. H., Choi, H. J., Tran, D., Abo, A., & Weis, W. I. (2011). Structural basis of Wnt signaling inhibition by Dickkopf binding to LRP5/6. Developmental cell, 21(5), 862-873.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0092CL | Anti-Human LRP6 Recombinant Antibody (77.2) | ELISA, FC, Inhib, FuncS, Internalization | |
TAB-0093CL | Anti-Human LRP6 Recombinant Antibody (135.16) | ELISA, FC, Inhib, FuncS, Internalization | |
TAB-0094CL | Anti-Human LRP6 Recombinant Antibody (213.7) | ELISA, FC, Inhib, FuncS, Internalization | |
TAB-0114CL-S(P) | Anti-Human LRP6 Recombinant Antibody scFv Fragment (1903.1) | ELISA, FC, Inhib, FuncS, Internalization | |
TAB-0348CL-F(E) | Human Anti-LRP6 Recombinant Antibody; Fab Fragment (TAB-0348CL-F(E)) | ELISA, FC, Inhib, FuncS, Internalization | Chimeric (mouse/human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0115CL | Chimeric Anti-Human LRP6 Recombinant Antibody | ELISA, FC, Inhib, FuncS, Internalization | Chimeric (mouse/human) |
TAB-0115CL-S(P) | Anti-Human LRP6 Recombinant Antibody scFv Fragment | ELISA, FC, Inhib, FuncS, Internalization | Chimeric (mouse/human) |
TAB-0115CL-F(E) | Anti-Human LRP6 Recombinant Antibody Fab Fragment | ELISA, FC, Inhib, FuncS, Internalization | Chimeric (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0705CL | Anti-Human LRP6 Recombinant Antibody (Fab004) | ELISA, FC, Inhib | Human antibody |
TAB-0706CL | Anti-Human LRP6 Recombinant Antibody (Fab002) | ELISA, FC, Inhib | Human antibody |
TAB-0707CL | Human Anti-LRP6 Recombinant Antibody (TAB-0707CL) | ELISA, FuncS, Inhib, Antagonist | Human IgG |
TAB-0708CL | Human Anti-LRP6 Recombinant Antibody (TAB-0708CL) | WB, Inhib, Block | Human IgG |
TAB-0705CL-F(E) | Anti-Human LRP6 Recombinant Antibody Fab Fragment (Fab004) | ELISA, FC | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0349CL-S(P) | Human Anti-LRP6 Recombinant Antibody; scFv Fragment (TAB-0349CL-S(P)) | ELISA | Humanized scFv |
TAB-0350CL-S(P) | Human Anti-LRP6 Recombinant Antibody; scFv Fragment (TAB-0350CL-S(P)) | ELISA | Humanized scFv |
TAB-0351CL-S(P) | Human Anti-LRP6 Recombinant Antibody; scFv Fragment (TAB-0351CL-S(P)) | ELISA | Humanized scFv |
TAB-0352CL-S(P) | Human Anti-LRP6 Recombinant Antibody; scFv Fragment (TAB-0352CL-S(P)) | ELISA | Humanized scFv |
TAB-0353CL-S(P) | Human Anti-LRP6 Recombinant Antibody; scFv Fragment (TAB-0353CL-S(P)) | ELISA | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBC-205 | Mouse Anti-LRP6 Recombinant Antibody (clone mAb135); scFv Fragment | ELISA, WB, FC | Mouse scFv |
HPAB-0648-WJ-S(P) | Human Anti-LRP6 Recombinant Antibody; scFv Fragment (HPAB-0648-WJ-S(P)) | ELISA | Human scFv |
HPAB-0649-WJ-S(P) | Human Anti-LRP6 Recombinant Antibody; scFv Fragment (HPAB-0649-WJ-S(P)) | ELISA | Human scFv |
HPAB-0650-WJ-S(P) | Human Anti-LRP6 Recombinant Antibody; scFv Fragment (HPAB-0650-WJ-S(P)) | ELISA | Human scFv |
HPAB-0651-WJ-S(P) | Human Anti-LRP6 Recombinant Antibody; scFv Fragment (HPAB-0651-WJ-S(P)) | ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2087 | Hi-Affi™ Recombinant Rabbit Anti-LRP6 Monoclonal Antibody (DS2087AB) | ICC/IF, WB | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0007-FY-F(E) | Human Anti-LRP6 Recombinant Antibody; Fab Fragment (HPAB-0007-FY-F(E)) | ELISA | Human Fab |
HPAB-0012-FY-F(E) | Human Anti-LRP6 Recombinant Antibody; Fab Fragment (HPAB-0012-FY-F(E)) | FuncS, Cyt | Human Fab |
HPAB-0647-WJ-F(E) | Human Anti-LRP6 Recombinant Antibody; Fab Fragment (HPAB-0647-WJ-F(E)) | ELISA | Human Fab |
HPAB-0648-WJ-F(E) | Human Anti-LRP6 Recombinant Antibody; Fab Fragment (HPAB-0648-WJ-F(E)) | ELISA | Human Fab |
HPAB-0649-WJ-F(E) | Human Anti-LRP6 Recombinant Antibody; Fab Fragment (HPAB-0649-WJ-F(E)) | ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0647-WJ | Human Anti-LRP6 Recombinant Antibody (HPAB-0647-WJ) | ELISA | Human IgG |
HPAB-0648-WJ | Human Anti-LRP6 Recombinant Antibody (HPAB-0648-WJ) | ELISA | Human IgG |
HPAB-0649-WJ | Human Anti-LRP6 Recombinant Antibody (HPAB-0649-WJ) | ELISA | Human IgG |
HPAB-0650-WJ | Human Anti-LRP6 Recombinant Antibody (HPAB-0650-WJ) | ELISA | Human IgG |
HPAB-0651-WJ | Human Anti-LRP6 Recombinant Antibody (HPAB-0651-WJ) | ELISA | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NBD-0060-YJ-VHH | Camelid Anti-LRP6 Recombinant Single Domain Antibody (L-P2-B10) | ELISA, Block | Camelid VHH |
NBD-0061-YJ-VHH | Camelid Anti-LRP6 Recombinant Single Domain Antibody (L-P2-D07) | ELISA, Block | Camelid VHH |
NBD-0062-YJ-VHH | Camelid Anti-LRP6 Recombinant Single Domain Antibody (L-P2-H07) | ELISA | Camelid VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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