Hi-Affi™ Recombinant Rabbit Anti-LRRC15 Monoclonal Antibody (DS2090AB)
CAT#: MOR-2090
Recombinant Rabbit anti-LRRC15 monoclonal antibody for WB, ICC/IF, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/412_C1_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/89507_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/89507_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/89507_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/89507_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/89507_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35503/89507_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Group enriched (fHDF/TERT166, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172061-LRRC15
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Specifications
- Immunogen
- Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS2090AB
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunocytochemistry/Immunofluorescence
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Consistent Performance in Oncology Research
This antibody demonstrates exceptional consistency across various assays. Its reproducibility is essential for reliable data, making it a preferred choice for researchers studying LRRC15 in oncology.
Effective in Immunocytochemistry
In immunocytochemistry applications, this antibody provides clear and specific staining. Its high affinity and specificity make it invaluable for detailed cell analysis in cancer studies.
High Specificity
The recombinant rabbit anti-LRRC15 monoclonal antibody exhibits superior specificity, ensuring minimal cross-reactivity. This is crucial for accurate identification of LRRC15 in complex biological samples.
Q&As
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What is the recommended dilution for using recombinant rabbit anti-LRRC15 monoclonal antibody in immunofluorescence?
A: For immunofluorescence applications, the recommended dilution is typically between 1:50 and 1:100. However, it is essential to optimize the dilution based on specific experimental conditions.
-
Can the recombinant rabbit anti-LRRC15 monoclonal antibody be used for immunoprecipitation?
A: Yes, the recombinant rabbit anti-LRRC15 monoclonal antibody can be used for immunoprecipitation. Incubate cell lysates with the antibody to form an antibody-antigen complex, capture it with protein A/G agarose beads, wash, and then elute the bound antigen for further analysis.
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What are the blocking conditions for using recombinant rabbit anti-LRRC15 monoclonal antibody in immunohistochemistry?
A: For immunohistochemistry, it is recommended to block non-specific binding sites using a buffer containing 5% BSA or normal serum. Incubate the tissue sections with the blocking buffer for at least 30 minutes before applying the primary antibody.
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How should the recombinant rabbit anti-LRRC15 monoclonal antibody be prepared for use in flow cytometry?
A: For flow cytometry, prepare single-cell suspensions and incubate them with the antibody at the recommended concentration. Use appropriate secondary antibodies and thoroughly wash the cells to reduce background staining before analysis on a flow cytometer.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "LRRC15"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-634 | Recombinant Anti-human LRRC15 Antibody | WB, IP, FuncS | IgG |
| MHH-634 | Recombinant Human Anti-human LRRC15 Antibody | WB, IF, FuncS | IgG |
| HPAB-0230-WJ | Human Anti-LRRC15 Recombinant Antibody (HPAB-0230-WJ) | ELISA, FC | Humanized IgG1 |
| HPAB-0231-WJ | Human Anti-LRRC15 Recombinant Antibody (HPAB-0231-WJ) | ELISA, FC | Humanized IgG1 |
| HPAB-0232-WJ | Human Anti-LRRC15 Recombinant Antibody (HPAB-0232-WJ) | ELISA, FC | Humanized IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-634-S(P) | Recombinant Anti-human LRRC15 Antibody scFv Fragment | Neut, WB, FuncS | scFv |
| MHH-634-S(P) | Recombinant Human Anti-human LRRC15 Antibody scFv Fragment | FC, IP, FuncS | scFv |
| HPAB-0230-WJ-S(P) | Human Anti-LRRC15 Recombinant Antibody; scFv Fragment (HPAB-0230-WJ-S(P)) | ELISA, FC | Humanized scFv |
| HPAB-0231-WJ-S(P) | Human Anti-LRRC15 Recombinant Antibody; scFv Fragment (HPAB-0231-WJ-S(P)) | ELISA, FC | Humanized scFv |
| HPAB-0232-WJ-S(P) | Human Anti-LRRC15 Recombinant Antibody; scFv Fragment (HPAB-0232-WJ-S(P)) | ELISA, FC | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0709CL | Anti-Human LRRC15 Recombinant Antibody | ELISA | |
| TAB-0709CL-S(P) | Anti-Human LRRC15 Recombinant Antibody scFv Fragment | ELISA | |
| TAB-0709CL-F(E) | Anti-Human LRRC15 Recombinant Antibody Fab Fragment | ELISA | |
| MOB-0318MC | Rabbit Anti-Human ALRRC15 polyclonal Antibody | WB, IHC-P, IF, IHC-P |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0230-WJ-F(E) | Human Anti-LRRC15 Recombinant Antibody; Fab Fragment (HPAB-0230-WJ-F(E)) | ELISA, FC | Humanized Fab |
| HPAB-0231-WJ-F(E) | Human Anti-LRRC15 Recombinant Antibody; Fab Fragment (HPAB-0231-WJ-F(E)) | ELISA, FC | Humanized Fab |
| HPAB-0232-WJ-F(E) | Human Anti-LRRC15 Recombinant Antibody; Fab Fragment (HPAB-0232-WJ-F(E)) | ELISA, FC | Humanized Fab |
| HPAB-0233-WJ-F(E) | Human Anti-LRRC15 Recombinant Antibody; Fab Fragment (HPAB-0233-WJ-F(E)) | ELISA, FC | Humanized Fab |
| HPAB-0234-WJ-F(E) | Human Anti-LRRC15 Recombinant Antibody; Fab Fragment (HPAB-0234-WJ-F(E)) | ELISA, FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY145 | Mouse Anti-LRRC15 scFv-Fc Chimera (VS-0425-FY145) | ELISA, FC | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC307 | Recombinant Anti-LRRC15 Vesicular Antibody, EV Displayed (VS-0425-YC307) | ELISA, FC, Cell-uptake |
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