Human Anti-LYZ Recombinant Antibody (clone dAb HEL4); scFv Fragment (CAT#: PSBW-022)
Recombinant Human Antibody (dAb HEL4) is capable of binding to Chicken LYZ, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Chicken LYZ scFv and CH1-3 region of human scFv and a light chain (LC) encoding VL from anti-Chicken LYZ scFv and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. HEL4 is highly soluble at concentrations of> or =3 mM, essentially monomeric and resistant to aggregation upon thermodenaturation at concentrations as high as 56 microM.
Host: Human
Isotype: Human scFv
Applications: ELISA
Specific Inquiry
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, the Golgi apparatus & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66182/1763_E3_3_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, the Golgi apparatus & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/66182/1763_E3_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human salivary gland
(High expression. RNA expression: 21115.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_1_val_selected.jpg
(High expression. RNA expression: 21115.5 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_1_val_selected.jpg
Figure 3 IHC staining of human skeletal muscle
(Low expression. RNA expression: 34.0 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_6_val_selected.jpg
(Low expression. RNA expression: 34.0 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_6_val_selected.jpg
Figure 4 Colon
(Mucosal lymphoid cells
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_3.jpg
(Mucosal lymphoid cells
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_3.jpg
Figure 5 Kidney
(Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_9_5.jpg
(Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_9_5.jpg
Figure 6 Testis
(Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55/231_A_5_6.jpg
(Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/55/231_A_5_6.jpg
Figure 7 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000090382-LYZ
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000090382-LYZ
Specifications
- Immunogen
- Chicken lysozyme (renal amyloidosis)
- Host Species
- Human
- Derivation
- Human
- Type
- Human scFv
- Specificity
- Chicken LYZ
- Species Reactivity
- Chicken
- Clone
- dAb HEL4
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- LYZ; lysozyme (renal amyloidosis); 1; LYZC; lysozyme C; dystrophin; allergen Gal d IV; egg white lysozyme; 4-beta-N-acetylmuramidase C; 1,4-beta-N-acetylmuramidase C
- Gene ID
- 396218
- UniProt ID
- P00698
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
| MOB-3387z | Mouse Anti-LYZ Recombinant Antibody (clone 39H8) | WB, FC, ICC, IF, IHC | Mouse IgG2a |
| PABL-429 | Human Anti-LYZ Recombinant Antibody | WB, IF, FuncS | Human IgG |
| MRO-0949-CN | Recombinant Rabbit Anti-LYZ Monoclonal Antibody (CBACN-349) | WB, IF, IHC, IP | Rabbit IgG |
| MOB-132YJL | Mouse Anti-LYZ Recombinant Antibody (clone muHYHEL-10) | WB, IHC, FuncS | Mouse IgG1, κ |
| MOB-111YJL | Human Anti-LYZ Recombinant Antibody (clone HYHEL-10 Y53D) | ELISA, FC, IHC | Human IgG1 |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
| PSBL-267 | Human Anti-LYZ Recombinant Antibody (clone HYHEL-10); scFv Fragment | WB, IHC, FuncS | Human scFv |
| PSBW-102 | Mouse Anti-LYZ Recombinant Antibody (clone D11.15); scFv Fragment | ELISA | Mouse scFv |
| MOB-111YJL-S(P) | Human Anti-LYZ Recombinant Antibody (clone HYHEL-10 Y53D); scFv Fragment | ELISA, FC, IHC | Human scFv |
| MOB-112YJL-S(P) | Human Anti-LYZ Recombinant Antibody (clone HYHEL-10 Y53D,Y58F); scFv Fragment | ELISA, FC, IHC | Human scFv |
| PSBS-0138 | Human Anti-LYZ Recombinant Antibody (Clone 01JF38); scFv Fragment | ELISA, FuncS | Human scFv |
Single-domain Antibody
| CAT | Product Name | Application | Type |
| PNBL-036 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (1D2L19) | WB, FuncS | Llama VHH |
| PNBL-051 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (cAbBCII-10) | ELISA | Llama VHH |
| PNBL-052 | Recombinant Anti-LYZ (D67H) VHH Single Domain Antibody (cAb-HuL6) | WB, ELISA, FuncS | Llama VHH |
| PNBL-053 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (cAb-Lys2) | WB, ELISA, FuncS | Llama VHH |
| PNBL-054 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (cAb-Lys3) | WB, FuncS | Llama VHH |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
| PFBZ-015 | Mouse Anti-LYZ Recombinant Antibody (clone HyHEL-5); Fab Fragment | WB, FuncS | Mouse Fab |
| PFBZ-022 | Mouse Anti-LYZ Recombinant Antibody (clone D44.1); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| PFBW-022 | Human Anti-LYZ Recombinant Antibody (clone dAb HEL4); Fab Fragment | ELISA | Human Fab |
| PFBC-023 | Mouse Anti-LYZ Recombinant Antibody (clone F10.6.6); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| PFBZ-023 | Mouse Anti-LYZ Recombinant Antibody (clone F9.13.7); Fab Fragment | WB, IHC, IF, FuncS | Mouse Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
| PABX-073 | Recombinant Human Anti-Lysozyme Antibody (HuLys) | WB, ELISA, FuncS | IgG |
| PABX-073-F (E) | Recombinant Human Anti-Lysozyme Antibody Fab Fragment (HuLys) | WB, ELISA, FuncS | Fab |
Human Antibody
| CAT | Product Name | Application | Type |
| PABX-138 | Recombinant Human Anti-Lysozyme Antibody | WB, Neut, FuncS | IgG |
| PABX-138-S (P) | Recombinant Human Anti-Lysozyme Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| PABX-139-S (P) | Mouse Anti-LYZ Recombinant Antibody; scFv Fragment (PABX-139-S (P)) | WB, ELISA, FuncS | Mouse scFv |
| PABX-138-F (E) | Recombinant Human Anti-Lysozyme Antibody Fab Fragment | WB, Neut, FuncS | Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
| PABX-139 | Mouse Anti-LYZ Recombinant Antibody (PABX-139) | WB, ELISA, FuncS | Mouse IgG |
| PABX-073-S (P) | Recombinant Human Anti-Lysozyme Antibody scFv Fragment (HuLys) | WB, ELISA, FuncS | scFv |
| PABX-074-S (P) | Recombinant Mouse Anti-Lysozyme Antibody scFv Fragment (HyHEL-5) | WB, ELISA, FuncS | scFv |
| PABX-074-F (E) | Recombinant Mouse Anti-Lysozyme Antibody Fab Fragment (HyHEL-5) | WB, ELISA, FuncS | Fab |
| PABX-139-F (E) | Mouse Anti-LYZ Recombinant Antibody; Fab Fragment (PABX-139-F (E)) | WB, ELISA, FuncS | Mouse Fab |
MHC Tetramer for Other Applications
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
| MOR-2112 | Hi-Affi™ Recombinant Rabbit Anti-LYZ Monoclonal Antibody (DS2112AB) | ICC, IHC-P, IP, WB | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
* Abbreviations
AgonistAgonist
BABioassay
InhibInhibition
PGProteogenomics
StimStimulation
WBWestern Blot
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