Mouse Anti-LYZ Recombinant Antibody (clone HYHEL-63) (CAT#: PABL-268)

Recombinant mouse antibody (HYHEL-63) is capable of binding to Lysozyme. The antibody was generated by immunizing with chicken lysozyme (renal amyloidosis).


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  • COA

Figure 1 (A) Binding of Fab HyHEL-63 to immobilized wild-type HEL. (B) Scatchard plot of the binding of Fab HyHEL-63 to wild-type HEL derived from the data depicted in panel A after correction for nonspecific binding. (C) Binding of Fab HyHEL-63 to the immobilized HEL K97A mutant. (D) Scatchard plot derived from the data depicted in panel C after correction for nonspecific binding.

Figure 1 (A) Binding of Fab HyHEL-63 to immobilized wild-type HEL. (B) Scatchard plot of the binding of Fab HyHEL-63 to wild-type HEL derived from the data depicted in panel A after correction for nonspecific binding. (C) Binding of Fab HyHEL-63 to the immobilized HEL K97A mutant. (D) Scatchard plot derived from the data depicted in panel C after correction for nonspecific binding.

(A) Fab HyHEL-63 was injected at 14 different concentrations ranging from 5.3 to 64 nM over a surface to which 550 RU of wild-type HEL had been coupled. Buffer flow rates were 5 µL/min. Equilibrium binding levels were reached within 50 min. After equilibrium had been reached, residual bound protein was eluted using a 1 min pulse of 10 mM HCl. (B) Req is the corrected equilibrium response at a given concentration C. The plot is linear with a correlation coefficient of 0.99. The apparent KA is 3.6 × 108 M⁻¹. The predicted maximum binding capacity (1900 RU) indicates that about 30% of the immobilized wild-type HEL molecules are available for binding. (C) Fab HyHEL-63 was injected at 10 different concentrations ranging from 0.1 to 3.2 µM over a surface to which 420 RU of HEL K97A had been coupled. (D) The plot is linear with a correlation coefficient of 0.99, and the apparent KA is 9.4 × 105 M⁻¹. Approximately 35% of the immobilized HEL K97A is available for binding as calculated from the predicted maximum binding capacity (1500 RU).

Li, Y., Li, H., Smith-Gill, S. J., & Mariuzza, R. A. (2000). Three-dimensional structures of the free and antigen-bound Fab from monoclonal antilysozyme antibody HyHEL-63.Biochemistry, 39(21), 6296-6309.

Figure 2 Pareto optimal and near-optimal HEL designs independently disrupting each antibody using 1 to 3 mutations.

Figure 2 Pareto optimal and near-optimal HEL designs independently disrupting each antibody using 1 to 3 mutations.

Choi, Y., Furlon, J. M., Amos, R. B., Griswold, K. E., & Bailey-Kellogg, C. (2018). DisruPPI: structure-based computational redesign algorithm for protein binding disruption.Bioinformatics, 34(13), i245-i253.


Specifications

  • Immunogen
  • Chicken lysozyme (renal amyloidosis)
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Chicken LYZ
  • Species Reactivity
  • Chicken
  • Clone
  • HYHEL-63
  • Applications
  • WB, ELISA, FuncS

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • LYZ; lysozyme (renal amyloidosis); 1; LYZC; lysozyme C; dystrophin; allergen Gal d IV; egg white lysozyme; 4-beta-N-acetylmuramidase C; 1,4-beta-N-acetylmuramidase C

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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See other products for "Clone HYHEL-63"

See other products for "LYZ"

Humanized Antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2112 Hi-Affi™ Recombinant Rabbit Anti-LYZ Monoclonal Antibody (DS2112AB) ICC, IHC-P, IP, WB IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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