Recombinant mouse antibody (HYHEL-63) is capable of binding to Lysozyme. The antibody was generated by immunizing with chicken lysozyme (renal amyloidosis).
Figure 1 (A) Binding of Fab HyHEL-63 to immobilized wild-type HEL. (B) Scatchard plot of the binding of Fab HyHEL-63 to wild-type HEL derived from the data depicted in panel A after correction for nonspecific binding. (C) Binding of Fab HyHEL-63 to the immobilized HEL K97A mutant. (D) Scatchard plot derived from the data depicted in panel C after correction for nonspecific binding.
(A) Fab HyHEL-63 was injected at 14 different concentrations ranging from 5.3 to 64 nM over a surface to which 550 RU of wild-type HEL had been coupled. Buffer flow rates were 5 µL/min. Equilibrium binding levels were reached within 50 min. After equilibrium had been reached, residual bound protein was eluted using a 1 min pulse of 10 mM HCl. (B) Req is the corrected equilibrium response at a given concentration C. The plot is linear with a correlation coefficient of 0.99. The apparent KA is 3.6 × 108 M⁻¹. The predicted maximum binding capacity (1900 RU) indicates that about 30% of the immobilized wild-type HEL molecules are available for binding. (C) Fab HyHEL-63 was injected at 10 different concentrations ranging from 0.1 to 3.2 µM over a surface to which 420 RU of HEL K97A had been coupled. (D) The plot is linear with a correlation coefficient of 0.99, and the apparent KA is 9.4 × 105 M⁻¹. Approximately 35% of the immobilized HEL K97A is available for binding as calculated from the predicted maximum binding capacity (1500 RU).
Li, Y., Li, H., Smith-Gill, S. J., & Mariuzza, R. A. (2000). Three-dimensional structures of the free and antigen-bound Fab from monoclonal antilysozyme antibody HyHEL-63.Biochemistry, 39(21), 6296-6309.
Figure 2 Pareto optimal and near-optimal HEL designs independently disrupting each antibody using 1 to 3 mutations.
Choi, Y., Furlon, J. M., Amos, R. B., Griswold, K. E., & Bailey-Kellogg, C. (2018). DisruPPI: structure-based computational redesign algorithm for protein binding disruption.Bioinformatics, 34(13), i245-i253.
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CAT | Product Name | Application | Type |
---|---|---|---|
PABL-267 | Human Anti-LYZ Recombinant Antibody (clone HYHEL-10) | WB, IHC, FuncS | Human IgG |
PABW-102 | Mouse Anti-LYZ Recombinant Antibody (clone D11.15) | ELISA | Mouse IgG |
PABL-429 | Human Anti-LYZ Recombinant Antibody | WB, IF, FuncS | Human IgG |
FAMAB-0212CQ | Recombinant Mouse Anti-LYZ Antibody (Hyb.C1) | ELISA | Mouse IgG |
PABS-0065 | Human Anti-LYZ Recombinant Single Domain Antibody (Clone H04) | ELISA, FuncS | Human VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
PNBL-023 | Recombinant Anti-Human LYZ VHH Single Domain Antibody (cAbHuL5) | WB, ELISA, FuncS | Llama VHH |
PNBL-024 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody () | WB, ELISA, FuncS | Llama VHH |
PNBL-036 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (1D2L19) | WB, FuncS | Llama VHH |
PNBL-051 | Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (cAbBCII-10) | ELISA | Llama VHH |
PNBL-052 | Recombinant Anti-LYZ (D67H) VHH Single Domain Antibody (cAb-HuL6) | WB, ELISA, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBZ-022 | Mouse Anti-LYZ Recombinant Antibody (clone D44.1); Fab Fragment | WB, IF, FuncS | Mouse Fab |
PFBW-022 | Human Anti-LYZ Recombinant Antibody (clone dAb HEL4); Fab Fragment | ELISA | Human Fab |
PFBC-023 | Mouse Anti-LYZ Recombinant Antibody (clone F10.6.6); Fab Fragment | WB, IF, FuncS | Mouse Fab |
PFBZ-023 | Mouse Anti-LYZ Recombinant Antibody (clone F9.13.7); Fab Fragment | WB, IHC, IF, FuncS | Mouse Fab |
PFBW-023 | Human Anti-LYZ Recombinant Antibody (clone HuLys11); Fab Fragment | WB, ELISA | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBW-102 | Mouse Anti-LYZ Recombinant Antibody (clone D11.15); scFv Fragment | ELISA | Mouse scFv |
FAMAB-0212CQ-S(P) | Recombinant Mouse Anti-LYZ Antibody scFv Fragment (Hyb.C1) | ELISA | Mouse scFv |
FAMAB-0213CQ-S(P) | Recombinant Mouse Anti-LYZ Antibody scFv Fragment (Gloop2) | ELISA | Mouse scFv |
FAMAB-0214CQ-S(P) | Recombinant Mouse Anti-LYZ Antibody scFv Fragment (Gloop3) | ELISA | Mouse scFv |
FAMAB-0215CQ-S(P) | Recombinant Mouse Anti-LYZ Antibody scFv Fragment (Gloop5) | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-073 | Recombinant Human Anti-Lysozyme Antibody (HuLys) | WB, ELISA, FuncS | IgG |
PABX-073-F (E) | Recombinant Human Anti-Lysozyme Antibody Fab Fragment (HuLys) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-074 | Recombinant Mouse Anti-Lysozyme Antibody (HyHEL-5) | WB, ELISA, FuncS | IgG |
PABX-139 | Mouse Anti-LYZ Recombinant Antibody (PABX-139) | WB, ELISA, FuncS | Mouse IgG |
PABX-073-S (P) | Recombinant Human Anti-Lysozyme Antibody scFv Fragment (HuLys) | WB, ELISA, FuncS | scFv |
PABX-074-S (P) | Recombinant Mouse Anti-Lysozyme Antibody scFv Fragment (HyHEL-5) | WB, ELISA, FuncS | scFv |
PABX-074-F (E) | Recombinant Mouse Anti-Lysozyme Antibody Fab Fragment (HyHEL-5) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-138 | Recombinant Human Anti-Lysozyme Antibody | WB, Neut, FuncS | IgG |
PABX-138-S (P) | Recombinant Human Anti-Lysozyme Antibody scFv Fragment | WB, Neut, FuncS | scFv |
PABX-139-S (P) | Mouse Anti-LYZ Recombinant Antibody; scFv Fragment (PABX-139-S (P)) | WB, ELISA, FuncS | Mouse scFv |
PABX-138-F (E) | Recombinant Human Anti-Lysozyme Antibody Fab Fragment | WB, Neut, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2112 | Hi-Affi™ Recombinant Rabbit Anti-LYZ Monoclonal Antibody (DS2112AB) | ICC, IHC-P, IP, WB | IgG |
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