Recombinant Mouse Anti-Lysozyme Antibody scFv Fragment (D1.3) (CAT#: PABX-139-S (P))

Anti-Lysozyme antibody is a Mouse of scFv class that binds to an Lysozyme.

  • Published Data
  • Datasheet
  • MSDS
  • COA

Figure 1 Representative images of spinal cord sections treated as in (A).

For each mAb, images with 8-18C5 or D1.3 pretreatment were acquired using the same settings and processed analogously. Alexa Fluor 555 and DAP1 fluorescence are pseudocolored red and green, respectively. Scale bar, 20 mm.

Bansal, P., Khan, T., Bussmeyer, U., Challa, D. K., Swiercz, R., Velmurugan, R., ... & Ward, E. S. (2013). The Encephalitogenic, Human Myelin Oligodendrocyte Glycoprotein–Induced Antibody Repertoire Is Directed toward Multiple Epitopes in C57BL/6-Immunized Mice. The Journal of Immunology, 191(3), 1091-1101.

Figure 2 A subset of the mAbs bind to mMOG expressed on the surface of transfected EL-4 cells.

EL4-MOG cells were incubated with 50 mg/ml mAb (black) or, as control, anti-lysozyme D1.3 Ab (mouse IgG1; gray). Bound Abs were detected using an Alexa 647–labeled anti-mouse IgG (H + L chain–specific) conjugate. Data are representative of three independent experiments.

Bansal, P., Khan, T., Bussmeyer, U., Challa, D. K., Swiercz, R., Velmurugan, R., ... & Ward, E. S. (2013). The Encephalitogenic, Human Myelin Oligodendrocyte Glycoprotein–Induced Antibody Repertoire Is Directed toward Multiple Epitopes in C57BL/6-Immunized Mice. The Journal of Immunology, 191(3), 1091-1101.

Figure 3 SPR assays to assess competition of mAbs with 8-18C5 for binding to recombinant mMOG.

mAb 8-18C5 and mMOG were immobilized on two flow cells of a CM5 sensor chip. mMOG (5 mg/ml; ∼200 nM) was injected (arrowhead) followed by mAb (arrow). Each mAb was injected at a concentration of ∼5-fold its Kd value for interaction with mMOG. Overlaid sensorgrams are shown for the 8-18C5 coupled flow cell (showing mMOG binding followed by mAb binding if the mAb does not compete with 8-18C5) and the mMOG coupled flow cell (showing only mAb binding as a control). The anti-lysozyme Ab D1.3 (35) was used as a control (bottom right panel). Data are representative of at least two injections for each mAb.

Bansal, P., Khan, T., Bussmeyer, U., Challa, D. K., Swiercz, R., Velmurugan, R., ... & Ward, E. S. (2013). The Encephalitogenic, Human Myelin Oligodendrocyte Glycoprotein–Induced Antibody Repertoire Is Directed toward Multiple Epitopes in C57BL/6-Immunized Mice. The Journal of Immunology, 191(3), 1091-1101.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • scFv
  • Specificity
  • Tested positive against native Human PD-L1
  • Species Reactivity
  • Hen
  • Clone
  • D1. 3
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:10000-1:20000
    FuncS (assay dependent)

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • LYZ; lysozyme (renal amyloidosis); 1; LYZC; lysozyme C; dystrophin; allergen Gal d IV; egg white lysozyme; 4-beta-N-acetylmuramidase C; 1,4-beta-N-acetylmuramidase C

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "LYZ"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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