Recombinant Mouse Anti-M. tuberculosis ESAT6 Antibody (HYB076-08) (CAT#: MRO-282CQ)

Figure 1 Expression of the Mtb fusion proteins in A549 cells infected with MVATG18377.

RpfB-RpfD-Ag85B-TB10.4-ESAT-6 fusion (*, expected molecular weight: 87.0 kDa) was detected using mouse monoclonal anti-ESAT-6 HYB076-08 antibody.

A Novel MVA-Based Multiphasic Vaccine for Prevention or Treatment of Tuberculosis Induces Broad and Multifunctional Cell-Mediated Immunity in Mice and Primates

Figure 2 The immunogenic components of RD1 are not expressed by the environmental mycobacteria M. avium, M. vaccae, and M. scrofulaceum.

Western blot analysis of antigens cross-reacting with an anti-ESAT-6 monoclonal antibody and with polyclonal anti-sera raised against CFP-10, PPE68 and Ag85B is shown.

Differential Effects of Prior Exposure to Environmental Mycobacteria on Vaccination with Mycobacterium bovis BCG or a Recombinant BCG Strain Expressing RD1 Antigens

Figure 3 Immunization with environmental mycobacteria generates cellular responses cross-reacting with the 30-kDa BCG antigen but not with ESAT-6.

Mycobacterium-specific IFN-γ production in mice (n = 3) infected with M. avium, M. scrofulaceum, M. vaccae, or a cocktail of the three strains (Mix) are compared. Splenocytes were stimulated in vitro with ₚESAT-6, Ag85B, or culture filtrate prepared from the relevant mycobacterial strain.

Differential Effects of Prior Exposure to Environmental Mycobacteria on Vaccination with Mycobacterium bovis BCG or a Recombinant BCG Strain Expressing RD1 Antigens

Figure 4 Specific cellular responses induced by BCG::RD1 vaccination are not altered by sensitization with environmental mycobacteria.

Mycobacterium-specific IFN-γ production in mice (n = 3) vaccinated with BCG::pYUB412 (BCG) or BCG::RD1 following sensitization with M. avium, M. scrofulaceum or M. vaccae is compared. Splenocytes were prepared 3 weeks postimmunization and stimulated in vitro with pESAT-6, Ag85B, or culture filtrates prepared from BCG or BCG::RD1 (BCG CF, BCG::RD1 CF).

Differential Effects of Prior Exposure to Environmental Mycobacteria on Vaccination with Mycobacterium bovis BCG or a Recombinant BCG Strain Expressing RD1 Antigens

Figure 5 Secretion of antigenic proteins by Mycobacterium tuberculosis and M. africanum.

Immunoblot of ESAT-6, CFP-10, and Ag85 family members in the culture filtrate (CF), comparing H37Rv, M. africanum GM041182, and the M. africanum Rv3879c complemented strain.

Impaired Fitness of Mycobacterium africanum Despite Secretion of ESAT-6