Human Anti-MAGEA3 Recombinant Antibody (clone M3H67) (CAT#: HPAB-0184-LSX)

Figure 1 21B4 vs M3H67 in MAG EA-Family-ELISA.

Serial dilutions of human-derived recombinant antibody 21B4 and murine mAb M3H67 were tested in ELISA for binding to MAGEAl, MAGEA3, MAGEA4 and MAGEA10. Antibodies were used in concentrations of 1000 ng/ml 1250 ng/ml, 312.5 ng/ml and 78 ng/ml. 21B4 binds specifically to MAGEA3, M3H67 shows preferential binding to MAGEA4, MAGE1 and MAGEA10. (Y-axis of graph in logarithmical scale.)

Figure 2 21B4 vs M3H67 in Western Blot Analysis.

Protein and MAGEA3 containing cell iysate of SK-MEL-37 cells was separated using Gradient SDS PAGE. Proteins were detected after blotting on nitro cellulose membrane using either 21B4 or mAb M3H67 (mg/ml each antibody). Bound antibody was detected using HRP-labelcd goat anti human IgG (21B4) or goat anti-mouse IgG (M3H67) secondary antibodies. Arrows mark position of MAGEA3.

Figure 3 IHC stainings of normal (non-cancerous) tissue using either 21B4rc or M3H67.

21B4rc shows a staining of distinct spermatogonia (arrow shows representative spermatogonia) of the testis whereas M3H67 stains all spermatogonia and the surrounding interstitial tissue. With Kidney and Pancreas no staining with either antibody is observed.

Figure 4 IHC stainings of cancerous tissue derived from head and neck cancers of three individual patients using either 21B4rc or M3H67.

Case 1 shows an overall similar staining by 21B4rc and M3H67. In Case 2, 21B4rc stains cancerous infiltrates which are not recognized by M3H67 and in case 3 a cancerous infiltrate recognized by M3H67 is not stained by 21B4rc.