Anti-Human MAGEA3 Recombinant Antibody (102G10)
CAT#: TAB-0718CL
Recombinant monoclonal antibody 102G10 is a human monoclonal antibody that specifically binds to human MAGEA3 and can be potentially used in the treatment of melanoma and breast cancer.
Published Data
Gene Expression
ELISA
Figure 1 Binding of recombinant human monoclonal antibodies to epitopes on MAGEA3.
Antibodies 102G10 were analyzed by ELISA using plates coated with overlapping peptides spanning the entire MAGEA3-protein.
WB
Figure 2 Recognition of MAGEA, but not of MAGEA4 by recombinant human monoclonal antibodies.
Human antibodies 102G10 were used to detect recombinant MAGEA3-(lane A) and recombinant MAGEA4-protein (lane B) in Western Blot analysis.
ELISA
Figure 3 EC50 determination of recombinant human monoclonal antibodies.
MAGEA3-ELISA using plates coated with recombinant MAGEA3 and serial dilutions of the human monoclonal antibodie 102G10 was carried out. EC50 values are indicated in the figure.
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RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (GAMG, K-562, OE19, PC-3, SH-SY5Y, SK-BR-3, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000221867-MAGEA3
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Specifications
- Host Species
- Human
- Derivation
- B cell
- Type
- Human antibody
- Antibody Isotype
- IgG1, κ
- Specificity
- Human
- Clone
- 102G10
- Applications
- ELISA, WB
- Related Disease
- Melanoma and breast cancer
Applications
- Application Notes
- The antibody was validated for ELISA, Western Blot. For details, refer to Published Data.
Target
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Specific for Human MAGEA3
The 102G10 recombinant antibody is highly specific for human MAGEA3. We used it in Western Blotting and obtained clean bands at the expected molecular weight. It is a valuable tool for our research into cancer/testis antigens and their expression in melanoma cell lines.
Excellent ELISA Results
We validated this human monoclonal antibody in our ELISA protocols. It showed high affinity binding to MAGEA3. The fact that it is a recombinant antibody produced in CHO cells gives us confidence in its stability and performance for our long-term studies.
Q&As
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What are the host species and production method for this antibody?
A: This is a recombinant human monoclonal antibody (clone 102G10), isotype IgG1, κ. It is produced in CHO cells to ensure high quality and consistency. The recombinant production platform avoids cell-line drift issues often associated with traditional methods.
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Which applications is this antibody recommended for?
A: The antibody 102G10 has been validated for use in Enzyme-linked Immunosorbent Assay (ELISA) and Western Blot (WB). It specifically binds to human MAGEA3 and is suitable for research into melanoma and breast cancer associated antigens.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 102G10"
- CAT
- Product Name
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| MHC-LC127 | PE-A*02:01/Human MAGEA3 (KVAELVHFL) MHC Tetramer | FCM |
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| HPAB-0183-LSX | Human Anti-MAGEA3 Recombinant Antibody (clone 21B4) | ELISA, WB, IP, FC, IHC | Human IgG |
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| HPAB-0183-LSX-S(P) | Human Anti-MAGEA3 Recombinant Antibody (clone 21B4); scFv Fragment | ELISA, WB | Human scFv |
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| HPAB-0184-LSX-S(P) | Human Anti-MAGEA3 Recombinant Antibody (clone M3H67); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
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| HPAB-0185-LSX-S(P) | Human Anti-MAGEA3 Recombinant Antibody (clone 31H10); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
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| HPAB-0183-LSX-F(E) | Human Anti-MAGEA3 Recombinant Antibody (clone 21B4); Fab Fragment | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
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| HPAB-0184-LSX-F(E) | Human Anti-MAGEA3 Recombinant Antibody (clone M3H67); Fab Fragment | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
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| HPAB-0185-LSX-F(E) | Human Anti-MAGEA3 Recombinant Antibody (clone 31H10); Fab Fragment | ELISA, WB | Human Fab |
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| HPAB-M0276-YC-S(P) | Human Anti-MAGEA3 Recombinant Antibody (clone M/YC-24); scFv Fragment | ELISA, WB | Humanized scFv |
| CAT | Product Name | Application | Type |
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| HPAB-M0276-YC-F(E) | Human Anti-MAGEA3 Recombinant Antibody (clone M/YC-24); Fab Fragment | ELISA, WB | Humanized Fab |
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