Mouse Anti-MAPT Recombinant Antibody (NS-090CN) (CAT#: NS-090CN)

Figure 1 Anti-MAPT Recombinant Antibody (NS-090CN) in DB

Dot Blot analysis of NS-090CN was performed by coating with human MAPT protein (His tag).
NS-090CN incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)

Figure 2 Anti-MAPT Recombinant Antibody (NS-090CN) in WB

Western blot analysis of NS-090CN was performed by loading human MAPT protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with NS-090CN and HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 3 Anti-MAPT Recombinant Antibody (NS-090CN) in ELISA

ELISA analysis of NS-090CN was performed by coating with human MAPT protein (His tag). Then blocked with BSA and incubated with NS-090CN. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.