Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH229DS)
CAT#: MOR-4715
Recombinant Rabbit anti-MAPT monoclonal antibody for WB, IF, ICC, IHC-P, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48895/1070_E6_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunohistochemical staining of human appendix shows strong positivity in peripheral ganglia and nerve fibers.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69524/156431_A_3_2_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining:
Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Proximal tubules (cell body)
Staining:
Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HDLM-2, SCLC-21H, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186868-MAPT
❮
❯
Specifications
- Immunogen
- Phosphopeptide corresponding to amino acids 224-236 of human microtubule-associated protein tau
- Host Species
- Rabbit
- Type
- IgG
- Species Reactivity
- Human, Rat
- Clone
- TH229DS
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunocytochemistry; Immunohistochemistry (Paraffin)
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Applications
- Application Notes
- ICC: 1:100-1:1000; IF: 1:100-1:1000; IHC-P: 1:50-1:500; WB: 1:500-1:5000
Target
- Alternative Names
- Microtubule Associated Protein Tau; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1; Microtubule-Associated Protein Tau; Neurofibrillary Tangle Protein; Paired Helical Filament-Tau; PHF-Tau; MAPTL; MTBT1; TAU; Protein Phosphatase 1, Regulatory Subunit 103; Regulatory Subunit 103; Protein Phosphatase 1; Isoform 4; PPP1R103; FTDP-17; MTBT2; DDPAC; MSTD; PPND
- Gene ID
- 4137
- UniProt ID
- P10636
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-674 | Chicken Anti-MAPT Recombinant Antibody (clone pT231/pS235_1); scFv Fragment | WB, ELISA, FACS, Block | Chicken scFv |
| PSBL-705 | Mouse Anti-MAPT Recombinant Antibody (clone DC8E8); scFv Fragment | WB, IF, FuncS | Mouse scFv |
| PSBL-706 | Rabbit Anti-MAPT Recombinant Antibody scFv Fragment (PSBL-706) | WB, IF, FuncS | Rabbit scFv |
| HPAB-0082-FY-S(P) | Mouse Anti-MAPT Recombinant Antibody; scFv Fragment (HPAB-0082-FY-S(P)) | WB, ELISA | Mouse scFv |
| HPAB-1104-CN-S(P) | Mouse Anti-MAPT Recombinant Antibody; scFv Fragment (HPAB-1104-CN-S(P)) | WB, ELISA, IF | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0733MZ | Chicken Anti-TAU Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-174 | Recombinant Mouse Anti-Tau5 Antibody | WB, ELISA, Neut, FuncS | IgG |
| PABX-174-S (P) | Recombinant Mouse Anti-Tau5 Antibody scFv Fragment | WB, ELISA, Neut, FuncS | scFv |
| PABX-174-F (E) | Recombinant Mouse Anti-Tau5 Antibody Fab Fragment | WB, ELISA, Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2164 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (DS2164AB) | IHC-P | IgG |
| MOR-4513 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH21DS) | WB, IHC-P, FC | IgG |
| MOR-4714 | Hi-Affi™ Recombinant Rabbit Anti-MAPT Monoclonal Antibody (TH228DS) | WB, IF, ICC, IHC-P, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-090CN | Mouse Anti-MAPT Recombinant Antibody (NS-090CN) | WB, ELISA, IHC, IP, SPR, FuncS | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1472-CN | Recombinant Rabbit Anti-MAPT Monoclonal Antibody (CBACN-546) | WB, IHC, IP | Rabbit IgG |
| MRO-1473-CN | Recombinant Rabbit Anti-MAPT Monoclonal Antibody (CBACN-547) | WB, IF, FC | Rabbit IgG |
| MRO-2209-CN | Rabbit Anti-MAPT Polyclonal Antibody (MRO-2209-CN) | WB, IF, IHC | Rabbit IgG |
| HPAB-AP145-YC | Human Anti-MAPT Recombinant Antibody (HPAB-AP145-YC) | ELISA | Human IgG1 |
| HPAB-0568-WJ | Mouse Anti-MAPT Recombinant Antibody (HPAB-0568-WJ) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-672-FY-F(E) | Mouse Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-672-FY-F(E)) | WB, ELISA | Mouse Fab |
| HPAB-679-FY-F(E) | Human Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-679-FY-F(E)) | WB, ELISA | Human Fab |
| HPAB-680-FY-F(E) | Mouse Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-680-FY-F(E)) | ELISA | Chimeric (human/mouse) Fab, λ |
| HPAB-0099-WJ-F(E) | Human Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-0099-WJ-F(E)) | ELISA, FC | Human Fab |
| HPAB-0106-WJ-F(E) | Human Anti-MAPT Recombinant Antibody; Fab Fragment (HPAB-0106-WJ-F(E)) | ELISA, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY182 | AbPlus™ Anti-MAPT Magnetic Beads (VS-0424-XY182) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY121 | Human Anti-MAPT (clone IPN002) scFv-Fc Chimera | ELISA, FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4255 | Anti-MAPT Immunohistochemistry Kit | IHC | |
| VS-0525-XY4256 | Anti-Mouse MAPT Immunohistochemistry Kit | IHC | |
| VS-0525-XY4257 | Anti-Rat MAPT Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC120 | Recombinant Anti-MAPT (AA 220-224 x AA 506-511) Biparatopic Antibody, Tandem scFv (Clone 13A6 x Clone 19G10) | ELISA | Tandem scFv |
| VS-0525-YC121 | Recombinant Anti-MAPT (AA 506-511 x AA 537-541) Biparatopic Antibody, Tandem scFv (Clone 19G10 x Clone 10H8) | ELISA | Tandem scFv |
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