Mouse Anti-MAPT Recombinant Antibody (NS-090CN) (CAT#: NS-090CN)
This product is a recombinant mouse antibody that recognizes MAPT. The recombinant human tau was used as an immunogen. The antibody has been tested in ELISA, Dot blot and western blot with human MAPT protein (His tag).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
DB
Figure 1 Anti-MAPT Recombinant Antibody (NS-090CN) in DB
Dot Blot analysis of NS-090CN was performed by coating with human MAPT protein (His tag).
NS-090CN incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)
WB
Figure 2 Anti-MAPT Recombinant Antibody (NS-090CN) in WB
Western blot analysis of NS-090CN was performed by loading human MAPT protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with NS-090CN and HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
ELISA
Figure 3 Anti-MAPT Recombinant Antibody (NS-090CN) in ELISA
ELISA analysis of NS-090CN was performed by coating with human MAPT protein (His tag). Then blocked with BSA and incubated with NS-090CN. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- Recombinant human tau
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human MAPT
- Species Reactivity
- Human
- Applications
- WB, ELISA, IHC, IP, SPR, FuncS
- Related Disease
- Neurodegenerative disorders
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
- Gene ID
- 4137
- UniProt ID
- P10636
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-305 | Chicken Anti-MAPT Recombinant Antibody; scFv Fragment | WB, ELISA, FuncS | Chicken scFv |
| PSBL-340 | Mouse Anti-MAPT Recombinant Antibody (clone AT8); scFv Fragment | ELISA, FuncS | Mouse scFv |
| HPAB-0099-WJ-S(P) | Human Anti-MAPT Recombinant Antibody; scFv Fragment (HPAB-0099-WJ-S(P)) | ELISA, FC | Human scFv |
| HPAB-0106-WJ-S(P) | Human Anti-MAPT Recombinant Antibody; scFv Fragment (HPAB-0106-WJ-S(P)) | ELISA, WB | Humanized scFv |
| HPAB-0108-WJ-S(P) | Rabbit Anti-MAPT Recombinant Antibody (clone Otau(S400)); scFv Fragment | ELISA | Rabbit scFv |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# NS-090CN, RRID: AB_3111573)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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