I'm studying Tau phosphorylation. Is this AT8 antibody appropriate for that?

Yes, the AT8 clone is a widely recognized tool for that specific purpose. This Anti-MAPT antibody (clone AT8) targets the phosphorylated form of the MAPT (Tau) protein. It is a well-established reagent in neurobiology research for studying tauopathies. This recombinant version is a mouse IgG1 isotype and has a purity of>95%.

What species and applications is this AT8 antibody (PABL-340) validated for?

This antibody shows broad species reactivity, making it very versatile. Our data lists reactivity with Human, Mouse, and Rat. It has also been validated for several key applications, including ELISA and Functional studies. This combination makes it a powerful reagent for comparing phosphorylated Tau in various models.

Mouse Anti-MAPT Recombinant Antibody (clone AT8)

CAT#: PABL-340

Recombinant mouse antibody (AT8) is capable of binding to Tau. The phospho-tau antibody AT8 has been tested in Immunocytochemistry in human AD brain and JNPL3 Tg mice.

Published Data Gene Expression

Figure 1 Immunocytochemistry of representative tau pathology in human AD brain and JNPL3 Tg mice with new antibodies 2D1 and 7F2.

Immuno-reactivity of previously characterized phospho-tau antibodies AT8 and new tau antibodies 2D1 or 7F2 in the hippocampus of a control individual or a subject with AD, and in the spinal cord of 12 month old nTg and JNPL3 Tg mice. Arrows indicating NFTs in human brain or NFT-like inclusion pathology in JNPL3 mice. Asterisk depicting dystrophic neurites within senile plaques. Bar = 100 μm, and 200 μm for insets

Strang, K. H., Goodwin, M. S., Riffe, C., Moore, B. D., Chakrabarty, P., Levites, Y.,... & Giasson, B. I. (2017). Generation and characterization of new monoclonal antibodies targeting the PHF1 and AT8 epitopes on human tau. Acta neuropathologica communications, 5(1), 58.

Figure 2 Characterization of the novel tau antibodies in detecting biochemically sarkosyl-insoluble tau in human brain lysates from AD patients.

Immunoblotting analysis of the sarkosyl-insoluble fraction from the temporal cortex of human AD cases (n = 3) and control cases (CTR; n = 2). Equal amounts of proteins (10 μg) from each sample was resolved onto 10% polyacrylamide gels and analyzed by immunoblotting with antibody indicated above blot

Figure 3 Analysis of tau signal with commercial monoclonal antibodies by Western blotting.

The proteins were extracted form the cortex of 3 mouse lines: control mice (WT and Hypothermic), Tau KO mice and 3xTg mice with n = 3 for each group. Proteins were extracted with SDS-PAGE and then identified with the following monoclonal antibody AT8. Normal anti-mouse (1) and Mouse TrueBlot ULTRA (2) secondary antibodies were used to detect primary antibodies.

Petry, F. R., Pelletier, J., Bretteville, A., Morin, F., Calon, F., Hébert, S. S.,... & Planel, E. (2014). Specificity of anti-tau antibodies when analyzing mice models of Alzheimer's disease: problems and solutions. PloS one, 9(5), e94251.

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Subcellular Location Figure 1 IF staining of human cell line BJ

Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48895/1070_E6_2_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line BJ

Immunohistochemical staining of human appendix shows strong positivity in peripheral ganglia and nerve fibers.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69524/156431_A_3_2_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Neuropil
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_B_8_5.jpg

Normal Tissue Figure 4 Colon

Endocrine cells
Staining:

Medium

Intensity: Moderate

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_3.jpg

Normal Tissue Figure 5 Kidney

Proximal tubules (cell body)
Staining:

Medium

Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_5.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (HDLM-2, SCLC-21H, T-47d)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186868-MAPT

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Specifications

Immunogen
Human microtubule-associated protein tau

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG

Specificity
Human MAPT

Species Reactivity
Human

Clone
AT8

Applications
ELISA, FuncS

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
MAPT; microtubule-associated protein tau; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103; PHF-tau; paired helical filament-tau; neurofibrillary tangle protein; microtubule-associated protein tau; isoform 4; protein phosphatase 1; regulatory subunit 103; G protein beta1/gamma2 subunit-interacting factor 1

Gene ID
4137

UniProt ID
P10636

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Great for Tau Research

This anti-MAPT antibody (clone AT8) is a classic reagent for neurobiology research, and this recombinant version is excellent. The data sheet lists its applications in ELISA and functional studies, which are all common assays for this target. The>95% purity is also a major plus.

Specific for Phospho-Tau

The AT8 clone is famous for its specificity, and this product delivers. It targets phosphorylated MAPT (Tau). The antibody is a mouse IgG1 isotype and is listed as reactive with human, making it highly useful for my phosphorylation studies.

Q&As

I'm studying Tau phosphorylation. Is this AT8 antibody appropriate for that?

A: Yes, the AT8 clone is a widely recognized tool for that specific purpose. This Anti-MAPT antibody (clone AT8) targets the phosphorylated form of the MAPT (Tau) protein. It is a well-established reagent in neurobiology research for studying tauopathies. This recombinant version is a mouse IgG1 isotype and has a purity of>95%.
What species and applications is this AT8 antibody (PABL-340) validated for?

A: This antibody shows broad species reactivity, making it very versatile. Our data lists reactivity with Human, Mouse, and Rat. It has also been validated for several key applications, including ELISA and Functional studies. This combination makes it a powerful reagent for comparing phosphorylated Tau in various models.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABL-340.pdf

MSDS

MSDS - PABL-340.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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