Mouse Anti-MERS-CoV S Recombinant Antibody (clone 4C2) (CAT#: PABL-324)
Recombinant Mouse Antibody (4C2) is capable of binding to S protein. This antibody could block virus entry in vitro with high efficacy.
Specific Inquiry
Figure 1 Interference of the spike/hCD26 interaction by the antibodies.
hCD26 was transiently expressed in BHK21 cells and tested by flow cytometry for MERS-RBD binding with or without the antibodies. Left to right: no antibody, with 2H8 (a non-neutralizing antibody targeting MERS-RBD), with 2E6, with 4C2.
Li, Y., Wan, Y., Liu, P., Zhao, J., Lu, G., Qi, J.,... & Zhang, B. (2015). A humanized neutralizing antibody against MERS-CoV targeting the receptor-binding domain of the spike protein.Cell research, 25(11), 1237.
Figure 2 In vitro neutralization tests using the pseudotyped and the live viruses.
The non-neutralizing antibody 2H8 or an irrelevant antibody L2 was used as the negative controls. Neutralization of the pseudoviruses by the antibodies (D). Neutralization of the live viruses by the antibodies (E).
Li, Y., Wan, Y., Liu, P., Zhao, J., Lu, G., Qi, J.,... & Zhang, B. (2015). A humanized neutralizing antibody against MERS-CoV targeting the receptor-binding domain of the spike protein.Cell research, 25(11), 1237.
Figure 3 Humanized 4C2 (4C2h) sustains similar neutralizing activity and biochemical characters to 4C2.
(A) SPR analysis of the MERS-RBD binding to 4C2h. The kinetic profile is shown. (B) Flow cytometric assay showing the blocking by 4C2h of the binding between MERS-RBD and hCD26 on the cell surface. (C, D) In vitro neutralization tests of 4C2h using the pseudotyped (C) and the live (D) viruses. The non-neutralizing antibody 2H8 or an irrelevant antibody L2 was used as the negative controls. The data are from four independent experiments, and mean ± SD is presented. (E, F) Protective efficacies of 4C2h in vivo. Ad5-hCD26-transduced BALB/c mice were treated intravenously with a single dose of antibody one day before (E) or after (F) the MERS-CoV challenge. Virus titers in the lungs were measured at 3 and 5 dpi and are expressed as PFUs per gram of lung tissue.
Li, Y., Wan, Y., Liu, P., Zhao, J., Lu, G., Qi, J.,... & Zhang, B. (2015). A humanized neutralizing antibody against MERS-CoV targeting the receptor-binding domain of the spike protein.Cell research, 25(11), 1237.
Specifications
- Immunogen
- MERS-CoV Spike glycoprotein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- MERS-CoV S protein
- Species Reactivity
- MERS-CoV
- Clone
- 4C2
- Applications
- WB, ELISA, Neut, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- S protein; Spike glycoprotein
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-325 | Mouse Anti-MERS-CoV S Recombinant Antibody (PABL-325) | Neut, FACS, ELISA, FuncS | Mouse IgG2a |
| PABL-326 | Human Anti-MERS-CoV S Recombinant Antibody (clone m336) | WB, Neut, FuncS | Human IgG1 |
| PABL-327 | Human Anti-MERS-CoV S Recombinant Antibody (clone MERS-27) | Neut, FuncS | Human IgG |
| PABC-274 | Recombinant Human Anti-MERS-CoV S Antibody (MCA1) | ELISA, Neut, FuncS | Human IgG |
| HPAB-0310-YJ | Human Anti-MERS-CoV S Recombinant Antibody (HPAB-0310-YJ) | ELISA, Neut, FuncS | Human IgG1, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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