Anti-Human MIF Recombinant Antibody (TAB-H41) (CAT#: TAB-H41)
Recombinant human antibody to Human MIF
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SDS-PAGE
Figure 1 Anti-Human MIF Recombinant Antibody (TAB-H41) in SDS-PAGE
SDS-PAGE analysis of TAB-H41 in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Anti-Human MIF Recombinant Antibody (TAB-H41) in SEC-HPLC
The purity of TAB-H41 was greater than 98% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
DB
Figure 3 Anti-Human MIF Recombinant Antibody (TAB-H41) in DB
Dot Blot analysis of TAB-H41 was performed by coating with human MIF protein (His tag).
TAB-H41-hIgG1 incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG Fc as a secondary antibody (1: 2000)
ELISA
Figure 4 Anti-Human MIF Recombinant Antibody (TAB-H41) in ELISA
ELISA analysis of TAB-H41 was performed by coating with human MIF protein (His tag). Then blocked with BSA and incubated with TAB-H41-hIgG1. The HRP conjugated Anti-human IgG Fc as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 5 Anti-Human MIF Recombinant Antibody (TAB-H41) in WB
WB analysis of TAB-H41 was performed by coating with human MIF protein (His tag).
TAB-H41 incubation concentration: 1 μg/mL.
HRP conjugated Anti-Human IgG Fc as a secondary antibody (1: 2000)
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
Specifications
- Host Species
- human
- Derivation
- human
- Type
- IgG1 - kappa
- Specificity
- MIF (macrophage migration inhibitory factor, GIF, GLIF, glycosylation-inhibiting factor) [Homo sapiens]
- Species Reactivity
- Human
- Applications
- Suitable for use in FuncS, IF, Neut, ELISA, FC, IP, IHC and most other immunological methods.
- Related Disease
- Cancers
Product Property
- Purity
- >95.0% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE.
Target
- Alternative Names
- imalumab;1430205-07-4;BAX69;MIF;macrophage migration inhibitory factor (glycosylation-inhibiting factor);GLIF;macrophage migration inhibitory factor;GIF;L-dopachrome isomerase;L-dopachrome tautomerase;phenylpyruvate tautomerase;MMIF;
- Gene ID
- 4282
- UniProt ID
- P14174
Related Resources
Please refer to Imalumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Imalumab.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1484z | Mouse Anti-MIF Recombinant Antibody (clone 19G7) | WB, ELISA, IHC | Mouse IgG1, κ |
| HPAB-1311-FY | Human Anti-MIF Recombinant Antibody (HPAB-1311-FY) | Inhib, ELISA | Human IgG |
| HPAB-1312-FY | Human Anti-MIF Recombinant Antibody (HPAB-1312-FY) | Inhib | Human IgG |
| HPAB-1313-FY | Human Anti-MIF Recombinant Antibody (HPAB-1313-FY) | Inhib | Human IgG |
| HPAB-1314-FY | Human Anti-MIF Recombinant Antibody (HPAB-1314-FY) | Inhib | Human IgG |
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