Anti-Human MMP9 Recombinant Antibody (TAB-002ML)
CAT#: TAB-002ML
Recombinant monoclonal antibody to MMP9. It is a human monoclonal antibody that can be potentially used in the treatment of Crohn's disease (CD), Colitis, ulcerative (UC), Solid tumors.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg
Normal Tissue
Figure 5 Testis
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
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SDS-PAGE
Figure 1 Anti-Human MMP9 Recombinant Antibody (TAB-002ML) in SDS-PAGE
SDS-PAGE analysis of TAB-002ML in non-reduced (Lane 2) and reduced (Lane 1) conditions.
HPLC
Figure 2 Anti-Human MMP9 Recombinant Antibody (TAB-002ML) in HPLC
HPLC analysis of TAB-002ML shows the purity is>95%.
ELISA
Figure 3 Anti-Human MMP9 Recombinant Antibody (TAB-002ML) in ELISA
ELISA analysis of TAB-002ML was performed by coating with Human MMP-9 Protein, His Tag (active enzyme).
The secondary antibody: Goat Anti-Human IgG(H+L)-HRP
WB
Figure 4 Anti-Human MMP9 Recombinant Antibody (TAB-002ML) in WB
Western blot analysis of TAB-002ML was performed with Human MMP-9 Protein, His Tag (active enzyme).
TAB-002ML incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Human IgG(H+L)-HRP
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
DB
Figure 5 Anti-Human MMP9 Recombinant Antibody (TAB-002ML) in DB
Dot Blot analysis of TAB-002ML was performed by coating with Human MMP-9 Protein, His Tag (active enzyme).
TAB-002ML incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Human IgG(H+L)-HRP
NC: Negative control PC: Positive control
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Chimeric
- Type
- IgG4, κ
- Specificity
- Human MMP9
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Immunofluorescence; Immunoprecipitation; Flow Cytometry; Functional Studies
- Conjugate
- Unconjugated
- Related Disease
- Crohn's disease (CD), Colitis, ulcerative (UC), Solid tumors
Product Property
- Purity
- > 95% as determined by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Enhanced Productivity in Research
The use of this antibody has greatly increased the efficacy of my research. Because of the good and dependable results, time and money are saved.
Lack of Reactivity in the Background
It looks that the antibody created a very good non-reactive background. It ensures that the data acquired is accurate and specific to the protein of interest.
Easy to Follow Instructions
The antibody is supplied with simple, straightforward protocols that ensure satisfactory experimental results without a great deal of troubleshooting.
Q&As
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What function do secondary antibodies serve in the process of detection?
A: Secondary antibodies provide more sensitive detection of the primary antibody's bound antigen by enhancing the signal. They frequently carry fluorophores or enzymes.
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How do I ensure that my sample will bind to antibodies specifically?
A: Titrate the protein of interest, use a range of controls, and do parallel tests with different antibodies that are aimed at to detect protein expression levels or concentrations.
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How can I improve the efficiency of the blocking stage in my protocols?
A: To lower background and increase signal specificity, test different blocking agents, such as BSA, serum, or commercial blocking buffers, and adjust concentrations and incubation times.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-002ML, RRID: AB_3111730)
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Biosimilar Overview
Please refer to Andecaliximab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Andecaliximab.
Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-533CL | Anti-Human MMP9 Recombinant Antibody (TAB-533CL) | WB, Inhib | Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0824CL | Human Anti-MMP9 Recombinant Antibody (TAB-0824CL) | ELISA | Human IgG4 |
| TAB-0825CL | Human Anti-MMP9 Recombinant Antibody (TAB-0825CL) | ELISA | Human IgG4 |
| TAB-0824CL-S(P) | Human Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0824CL-S(P)) | ELISA | Human scFv |
| TAB-0825CL-S(P) | Human Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0825CL-S(P)) | ELISA | Human scFv |
| TAB-0824CL-F(E) | Human Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0824CL-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0826CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0826CL) | Inhib, ELISA, IHC | Mouse IgG |
| TAB-0827CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0827CL) | ELISA | Mouse IgG |
| TAB-0826CL-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0826CL-S(P)) | ELISA | Mouse scFv |
| TAB-0826CL-F(E) | Mouse Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0826CL-F(E)) | ELISA | Mouse Fab |
| TAB-0827CL-F(E) | Mouse Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0827CL-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-174 | Mouse Anti-MMP9 Recombinant Antibody Fab Fragment (PFBC-174) | ELISA, Neut | Mouse Fab |
| HPAB-0027-YC-F(E) | Mouse Anti-MMP9 Recombinant Antibody; Fab Fragment (HPAB-0027-YC-F(E)) | FC | Mouse Fab |
| HPAB-0028-YC-F(E) | Human Anti-MMP9 Recombinant Antibody (clone G03-VH-GL-V1/ G06-VL-GL1); Fab Fragment | Neut | Human Fab |
| HPAB-0513LY-F(E) | Mouse Anti-MMP9 Recombinant Antibody (clone M4); Fab Fragment | FC | Mouse Fab |
| HPAB-0514LY-F(E) | Mouse Anti-MMP9 Recombinant Antibody (clone M12); Fab Fragment | FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0717MZ | Chicken Anti-MMP9 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1748CQ | Recombinant Mouse Anti-MMP9 Antibody (VMC204) | Inhib, IHC-Fr, IP, IHC-P, WB, IHC, ICC | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2263 | Hi-Affi™ Recombinant Rabbit Anti-MMP9 Monoclonal Antibody (DS2263AB) | FC, IHC-P, WB | IgG |
| MOR-4331 | Hi-Affi™ Recombinant Rabbit Anti-MMP-9 Monoclonal Antibody (SI420DS) | ELISA | IgG |
| MOR-4332 | Hi-Affi™ Recombinant Rabbit Anti-MMP-9 Monoclonal Antibody (SI421DS) | ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0027-YC-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (HPAB-0027-YC-S(P)) | FC | Mouse scFv |
| HPAB-0028-YC-S(P) | Human Anti-MMP9 Recombinant Antibody (clone G03-VH-GL-V1/ G06-VL-GL1); scFv Fragment | Neut | Human scFv |
| HPAB-0199-FY-S(P) | Human Anti-MMP9 Recombinant Antibody (clone DX-2802); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-0200-FY-S(P) | Human Anti-MMP9 Recombinant Antibody (clone M0279-A03); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-1320-FY-F(E) | Human Anti-MMP9 Recombinant Antibody; Fab Fragment (HPAB-1320-FY-F(E)) | Activ | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2039-CN | Rabbit Anti-MMP9 Polyclonal Antibody (MRO-2039-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS-0522-LC77 | Mouse Anti-MMP9 Recombinant Antibody (VS-0522-LC77) | WB, IF, IHC | Mouse IgG2a |
| VS-0522-LC78 | Rat Anti-MMP9 Recombinant Antibody (VS-0522-LC78) | WB, IF, IHC | Rat IgG2b |
| VS-0522-LC79 | Human Anti-MMP9 Recombinant Antibody (VS-0522-LC79) | WB, IF, IHC | Human IgG1 |
| VS-0522-LC80 | Rabbit Anti-MMP9 Recombinant Antibody (VS-0522-LC80) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-002ML | Afuco™ Anti-MMP9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-002ML) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX783 | Mouse Anti-MMP9 Recombinant Antibody (clone 331CT17.4.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY101 | Human Anti-MMP9 (clone DX-2802) scFv-Fc Chimera | WB, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1357 | Anti-MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4460 | Anti-Mouse MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4461 | Anti-Zebrafish MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4459 | Anti-Human MMP9 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC735 | CytoStream™ Rabbit Anti-MMP9 Recombinant Antibody (VS13-YC735) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
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