Anti-Human MMP9 Recombinant Antibody (TAB-533CL)
CAT#: TAB-533CL
Recombinant Humanized monoclonal antibody expressed in CHO binding to Human MMP9. This antibody is a monoclonal antibody that inhibits matrix metalloproteinase 9 (MMP9), an extracellular enzyme involved in matrix remodeling, tumor growth, and metastasis. Inhibiting MMP9 is expected to block paracrine signaling and metastasis and to alter the immune environment within the tumor.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg
Normal Tissue
Figure 5 Testis
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
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SDS-PAGE
Figure 1 Anti-Human MMP9 Recombinant Antibody (TAB-533CL) in SDS-PAGE
SDS-PAGE analysis of TAB-533CL in non-reduced (Lane 1) and reduced (Lane 2) conditions.
HPLC
Figure 2 Anti-Human MMP9 Recombinant Antibody (TAB-533CL) in HPLC
HPLC analysis of TAB-533CL shows the purity is>95%.
ELISA
Figure 3 Anti-Human MMP9 Recombinant Antibody (TAB-533CL) in ELISA
ELISA analysis of TAB-533CL was performed by coating with Human MMP-9 Protein, His Tag (active enzyme).
The secondary antibody: Goat Anti-Human IgG(H+L)-HRP
WB
Figure 4 Anti-Human MMP9 Recombinant Antibody (TAB-533CL) in WB
Western blot analysis of TAB-533CL was performed with Human MMP-9 Protein, His Tag (active enzyme).
TAB-533CL incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Human IgG(H+L)-HRP
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
DB
Figure 5 Anti-Human MMP9 Recombinant Antibody (TAB-533CL) in DB
Dot Blot analysis of TAB-533CL was performed by coating with Human MMP-9 Protein, His Tag (active enzyme).
TAB-533CL incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Human IgG(H+L)-HRP
NC: Negative control PC: Positive control
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Specifications
- Derivation
- Humanized
- Type
- Antibody
- Species Reactivity
- Human
- Applications
- WB, Inhib
- Related Disease
- Solid Tumors
Product Property
- Purity
- >95%, by SDS-PAGE with Coomassie Brilliant Blue staining
- Storage
- ≤1 year at -20°C.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot and Inhibition. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- MMP9; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); GELB; CLG4B; MMP-9; MANDP2; matrix metalloproteinase-9; type V collagenase; macrophage gelatinase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92k
- Gene ID
- 4318
- UniProt ID
- P14780
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1048 | Recombinant Mouse Anti-Human MMP9 Antibody Fab Fragment | LMNX, ELISA, WB, IF | Fab |
| HPAB-0513LY-F(E) | Mouse Anti-MMP9 Recombinant Antibody (clone M4); Fab Fragment | FC | Mouse Fab |
| HPAB-0514LY-F(E) | Mouse Anti-MMP9 Recombinant Antibody (clone M12); Fab Fragment | FC | Mouse Fab |
| HPAB-M0359-YC-F(E) | Human Anti-MMP9 Recombinant Antibody (clone AB0045); Fab Fragment | ELISA | Humanized Fab |
| HPAB-1320-FY-S(P) | Human Anti-MMP9 Recombinant Antibody; scFv Fragment (HPAB-1320-FY-S(P)) | Activ | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0824CL | Human Anti-MMP9 Recombinant Antibody (TAB-0824CL) | ELISA | Human IgG4 |
| TAB-0825CL | Human Anti-MMP9 Recombinant Antibody (TAB-0825CL) | ELISA | Human IgG4 |
| TAB-0824CL-S(P) | Human Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0824CL-S(P)) | ELISA | Human scFv |
| TAB-0824CL-F(E) | Human Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0824CL-F(E)) | ELISA | Human Fab |
| TAB-0825CL-F(E) | Human Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0825CL-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0826CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0826CL) | Inhib, ELISA, IHC | Mouse IgG |
| TAB-0827CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0827CL) | ELISA | Mouse IgG |
| TAB-0826CL-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0826CL-S(P)) | ELISA | Mouse scFv |
| TAB-0827CL-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0827CL-S(P)) | ELISA | Mouse scFv |
| TAB-0826CL-F(E) | Mouse Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0826CL-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-174 | Mouse Anti-MMP9 Recombinant Antibody scFv Fragment (PSBC-174) | ELISA, Neut | Mouse scFv |
| HPAB-0199-FY-S(P) | Human Anti-MMP9 Recombinant Antibody (clone DX-2802); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-0200-FY-S(P) | Human Anti-MMP9 Recombinant Antibody (clone M0279-A03); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-0513LY-S(P) | Mouse Anti-MMP9 Recombinant Antibody (clone M4); scfv Fragment | FC | Mouse scfv |
| HPAB-0514LY-S(P) | Mouse Anti-MMP9 Recombinant Antibody (clone M12); scfv Fragment | FC | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0717MZ | Chicken Anti-MMP9 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1748CQ | Recombinant Mouse Anti-MMP9 Antibody (VMC204) | Inhib, IHC-Fr, IP, IHC-P, WB, IHC, ICC | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2263 | Hi-Affi™ Recombinant Rabbit Anti-MMP9 Monoclonal Antibody (DS2263AB) | FC, IHC-P, WB | IgG |
| MOR-4331 | Hi-Affi™ Recombinant Rabbit Anti-MMP-9 Monoclonal Antibody (SI420DS) | ELISA | IgG |
| MOR-4332 | Hi-Affi™ Recombinant Rabbit Anti-MMP-9 Monoclonal Antibody (SI421DS) | ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-002ML | Anti-Human MMP9 Recombinant Antibody (TAB-002ML) | ELISA, IHC, FC, IP, IF, FuncS | IgG4, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-FY | Human Anti-MMP9 Recombinant Antibody (clone DX-2802) | ELISA, FC | Human IgG1, λ |
| HPAB-0200-FY | Human Anti-MMP9 Recombinant Antibody (clone M0279-A03) | ELISA, FC | Human IgG |
| MRO-1014-CN | Recombinant Rabbit Anti-MMP9 Monoclonal Antibody (JA80-73) | WB, IF, IHC, FC | Rabbit IgG |
| VS7-0425-WR402 | Mouse Anti-MMP9 Monoclonal Antibody (VS7-0425-WR402) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
| VS7-0425-WR403 | Mouse Anti-MMP9 Monoclonal Antibody (VS7-0425-WR403) | WB, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-002ML | Afuco™ Anti-MMP9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-002ML) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX783 | Mouse Anti-MMP9 Recombinant Antibody (clone 331CT17.4.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY101 | Human Anti-MMP9 (clone DX-2802) scFv-Fc Chimera | WB, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1357 | Anti-MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4460 | Anti-Mouse MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4461 | Anti-Zebrafish MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4459 | Anti-Human MMP9 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC735 | CytoStream™ Rabbit Anti-MMP9 Recombinant Antibody (VS13-YC735) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
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