Recombinant Mouse Anti-MSP2 Antibody (6D8) (CAT#: PABL-659)

Recombinant Mouse Antibody (6D8) is capable of binding to MSP2 , expressed in Chinese Hamster Ovary cells (CHO).

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Figure 1 MSP2 may be polymeric on the merozoite surface.

Figure 1 MSP2 may be polymeric on the merozoite surface.

Binding of monoclonal antibodies 6D8 to monomeric (triangles) MSP2 as shown by ELISA.

Adda, C. G., Murphy, V. J., Sunde, M., Waddington, L. J., Schloegel, J., Talbo, G. H., ... & Hodder, A. N. (2009). Plasmodium falciparum merozoite surface protein 2 is unstructured and forms amyloid-like fibrils. Molecular and biochemical parasitology, 166(2), 159-171.

Figure 2 MSP2 may be polymeric on the merozoite surface.

Figure 2 MSP2 may be polymeric on the merozoite surface.

Western blotting experiments of recombinant 3D7 (lane 1) and FC27 MSP2 (lane 2), the PK-resistant core (lane 3), and 3D7 (lane 4) and D10 (lane 5) parasite lysates probed with mAbs 6D8 and 11E1 and polyclonal sheep sera raised against 3D7 and FC27 MSP2.

Adda, C. G., Murphy, V. J., Sunde, M., Waddington, L. J., Schloegel, J., Talbo, G. H., ... & Hodder, A. N. (2009). Plasmodium falciparum merozoite surface protein 2 is unstructured and forms amyloid-like fibrils. Molecular and biochemical parasitology, 166(2), 159-171.

Figure 3 MSP2 may be polymeric on the merozoite surface.

Figure 3 MSP2 may be polymeric on the merozoite surface.

Immunofluorescence microscopy data showing mAb11E1 binding to the 3D7 parasite strain only (f, fluorescence; n, nuclear stain; b, bright field).

Adda, C. G., Murphy, V. J., Sunde, M., Waddington, L. J., Schloegel, J., Talbo, G. H., ... & Hodder, A. N. (2009). Plasmodium falciparum merozoite surface protein 2 is unstructured and forms amyloid-like fibrils. Molecular and biochemical parasitology, 166(2), 159-171.


Specifications

  • Immunogen
  • P. falciparum merozoite surface protein 2 precursor
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native MSP2
  • Species Reactivity
  • P. falciparum
  • Clone
  • 6D8
  • Applications
  • WB, IF, FuncS

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The MSP2 antibody has been reported in applications of WB, ELISA, IF.
    WB: 6D8 can be used to detect recombinant FC27 and 3D7 MSP2 which has been subjected to SDS-PAGE under non-reducing conditions. Parasite FC27 MSP reactivity with 6D8 is enhanced in western blots when carried out in reducing conditions.
    IF: 6D8 can be used to detect MSP2 by immunofluorescence.

Target

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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