Mouse Anti-MMP14 Recombinant Antibody (clone LEM-2/15) (CAT#: PABL-279)

Recombinant mouse antibody (LEM-2/15) is capable of binding to MT1-MMP. The antibody LEM-2/15 was generated by immunizing mice with a peptide possessing the sequence of the V-B loop (aa 218-233).


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA

Figure 1 Immunohistochemistry with the LEM2/15 anti MT1-MMP antibody in a TMA (n = 40) of Low-grade GBMs (LGG) and High-grade GBMs (HGG) patient tissues (representative images). Right panel: MT1-MMP staining intensity score (0–3).

Figure 1 Immunohistochemistry with the LEM2/15 anti MT1-MMP antibody in a TMA (n = 40) of Low-grade GBMs (LGG) and High-grade GBMs (HGG) patient tissues (representative images). Right panel: MT1-MMP staining intensity score (0–3).

de Lucas, A. G., Schuhmacher, A. J., Oteo, M., Romero, E., Cámara, J. A., de Martino, A.,... & Mulero, F. (2016). Targeting MT1-MMP as an ImmunoPET-based strategy for imaging gliomas. PloS one, 11(7), e0158634.

Figure 2 Immunoblotting analyses of the indicated cell lines using the LEM2/15 anti MT1-MMP antibody.

Figure 2 Immunoblotting analyses of the indicated cell lines using the LEM2/15 anti MT1-MMP antibody.

de Lucas, A. G., Schuhmacher, A. J., Oteo, M., Romero, E., Cámara, J. A., de Martino, A.,... & Mulero, F. (2016). Targeting MT1-MMP as an ImmunoPET-based strategy for imaging gliomas. PloS one, 11(7), e0158634.

Figure 3 FACS analyses of the indicated cell lines using the LEM2/15 anti MT1-MMP antibody.

Figure 3 FACS analyses of the indicated cell lines using the LEM2/15 anti MT1-MMP antibody.

de Lucas, A. G., Schuhmacher, A. J., Oteo, M., Romero, E., Cámara, J. A., de Martino, A.,... & Mulero, F. (2016). Targeting MT1-MMP as an ImmunoPET-based strategy for imaging gliomas. PloS one, 11(7), e0158634.

Figure 4 Immunohistochemistry of tumor tissue from xenografted mice used for PET imaging. MT1-MMP was detected using LEM2/15 antibody. Scale bars: 500 μm.

Figure 4 Immunohistochemistry of tumor tissue from xenografted mice used for PET imaging. MT1-MMP was detected using LEM2/15 antibody. Scale bars: 500 μm.

de Lucas, A. G., Schuhmacher, A. J., Oteo, M., Romero, E., Cámara, J. A., de Martino, A.,... & Mulero, F. (2016). Targeting MT1-MMP as an ImmunoPET-based strategy for imaging gliomas. PloS one, 11(7), e0158634.

Figure 5 PET/CT imaging with radiolabeled 89Zr-DFO-LEM2/15 in mice bearing orthotopic xenografts containing patient-derived neurospheres.

Figure 5 PET/CT imaging with radiolabeled 89Zr-DFO-LEM2/15 in mice bearing orthotopic xenografts containing patient-derived neurospheres.

A) Representative fused PET/CT images of coronal and sagittal planes at 2 and 4 days p.i. containing TS543 brain tumors with 89Zr-DFO-LEM2/15 (left panels) and 89Zr-DFO-IgG1 as isotype control (right panels) B) Tumor-to-blood ratios for 89Zr-DFO-LEM2/15 (black bars) and 89Zr-DFO-IgG1 (grey bars) in nude mice bearing orthotopic TS453 xenografts at 2 and 4 days p.i. derived from PET imaging.

de Lucas, A. G., Schuhmacher, A. J., Oteo, M., Romero, E., Cámara, J. A., de Martino, A.,... & Mulero, F. (2016). Targeting MT1-MMP as an ImmunoPET-based strategy for imaging gliomas. PloS one, 11(7), e0158634.

Figure 6 ANS fluorescence assay. ANS (250 mM) was added to CAT-MT1-MMP (5 mM), Fab LEM-2/15 (5 mM), and a 1:1 complex. The samples were excited at 350 nm, and fluorescence spectra (400–600 nm) were recorded. The fluorescence intensity was calculated as the area of the spectra at 400–600 nm (ANS, black; MT1- MMP, red; Fab LEM-2/15, green; MT1-MMP:Fab LEM-2/15, blue).

Figure 6 ANS fluorescence assay. ANS (250 mM) was added to CAT-MT1-MMP (5 mM), Fab LEM-2/15 (5 mM), and a 1:1 complex. The samples were excited at 350 nm, and fluorescence spectra (400–600 nm) were recorded. The fluorescence intensity was calculated as the area of the spectra at 400–600 nm (ANS, black; MT1- MMP, red; Fab LEM-2/15, green; MT1-MMP:Fab LEM-2/15, blue).

The inset shows emission spectra of the proteins in the presence of ANS (ANS, black; MT1- MMP, red; Fab LEM-2/15, green; MT1-MMP:Fab LEM-2/15, blue).

Udi, Y., Grossman, M., Solomonov, I., Dym, O., Rozenberg, H., Moreno, V.,... & Sagi, I. (2015). Inhibition mechanism of membrane metalloprotease by an exosite-swiveling conformational antibody. Structure, 23(1), 104-115.


Specifications

  • Immunogen
  • A peptide possessing the sequence of the V-B loop, residues 218-233.
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human MMP14
  • Species Reactivity
  • Human
  • Clone
  • LEM-2/15
  • Applications
  • WB, ELISA, FuncS

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • MMP14; matrix metallopeptidase 14 (membrane-inserted); MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1; matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type-1 matrix metalloproteinase

Related Resources

  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone LEM-2/15"

See other products for "MMP14"

Single-domain Antibody

CAT Product Name Application Type
NAB-865-VHH Recombinant Anti-human MMP14 VHH Single Domain Antibody WB, ICC, ChiP, FA, ELISA Llama VHH

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0370MZ Chicken Anti-MMP14 Polyclonal IgY WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2251 Hi-Affi™ Recombinant Rabbit Anti-MMP14 Monoclonal Antibody (DS2251AB) IHC-P, ICC/IF, FC, IP, WB IgG

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for PABL-279. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare