Humanized Anti-MUC1 Recombinant Antibody (clone Cantuzumab) (CAT#: TAB-166)

This product is a humanized (from mouse) IgG1, κ antibody that can recognize human MUC1.


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ELISA

Figure 1 Clearance of huC242-DM1 conjugate and its huC242 antibody component as measured by ELISA methods in the plasma of CD-1 mice after intravenous administration of 10 mg/kg conjugate having, on the average, 3.2 DM1 molecules linked per antibody molecule.

Figure 1 Clearance of huC242-DM1 conjugate and its huC242 antibody component as measured by ELISA methods in the plasma of CD-1 mice after intravenous administration of 10 mg/kg conjugate having, on the average, 3.2 DM1 molecules linked per antibody molecule.

Each data point is the mean S.D. of the measurements of three samples from three different animals.

Xie, H., Audette, C., Hoffee, M., Lambert, J. M., & Blättler, W. A. (2004). Pharmacokinetics and biodistribution of the antitumor immunoconjugate, cantuzumab mertansine (huC242-DM1), and its two components in mice. Journal of Pharmacology and Experimental Therapeutics, 308(3), 1073-1082.

ELISA

Figure 2 ELISA result.

Figure 2 ELISA result.

A, clearance of huC242-[3H]DM1 from the serum of CD-1 mice that had been injected i.v. with 3.93 mg/kg huC242-[3H]DM1. The conjugate contained, on the average, 3.2 linked [3H]DM1 drugs per antibody molecule. The clearance was measured either by counting the radioactivity (E) associated with the serum samples or by ELISA for the antibody component (F) of the conjugate. B, change in the number of DM1 drugs linked per huC242 antibody molecule during circulation in CD-1 mice. For each time point of the clearance curves in Fig, the number of DM1 molecules linked per huC242 molecule was calculated using the known specific molar radioactivity of [3H]DM1 and a Mr of 147 kDa for huC242 and was then plotted as logarithm versus the time after intravenous administration of the huC242-[3H]DM1 conjugate.

Xie, H., Audette, C., Hoffee, M., Lambert, J. M., & Blättler, W. A. (2004). Pharmacokinetics and biodistribution of the antitumor immunoconjugate, cantuzumab mertansine (huC242-DM1), and its two components in mice. Journal of Pharmacology and Experimental Therapeutics, 308(3), 1073-1082.

PK

Figure 3 Pharmacokinetics and Pharmacodynamics result.

Figure 3 Pharmacokinetics and Pharmacodynamics result.

A representative patient's plasma cantuzumab mertansine concentration versus time curve for courses 1 and 5 at the 235-mg/m² dose level.

Tolcher, A. W., Ochoa, L., Hammond, L. A., Patnaik, A., Edwards, T., Takimoto, C.,... & Jonak, Z. L. (2003). Cantuzumab mertansine, a maytansinoid immunoconjugate directed to the CanAg antigen: a phase I, pharmacokinetic, and biologic correlative study. Journal of clinical oncology, 21(2), 211-222.

IHC

Figure 4 Immunohistochemical result.

Figure 4 Immunohistochemical result.

Immunohistochemical detection for (A) CanAg expression (2 homogeneous), (B) human IgG, and (C) DM1-huC242 (cantuzumab mertansine) in the tumor biopsy obtained 24 hours after the first infusion of cantuzumab mertansine.

Tolcher, A. W., Ochoa, L., Hammond, L. A., Patnaik, A., Edwards, T., Takimoto, C.,... & Jonak, Z. L. (2003). Cantuzumab mertansine, a maytansinoid immunoconjugate directed to the CanAg antigen: a phase I, pharmacokinetic, and biologic correlative study. Journal of clinical oncology, 21(2), 211-222.

PK

Figure 5 Clearance of 125I-huC242 antibody (F) and 125I-huC242-DM1 conjugate (E) from the blood of mice that had been injected intravenously either with 4.16 mg/kg 125I-huC242 or 4.16 mg/kg 125I-huC242-DM1 conjugate that had, on the average, 4.07 DM1 drugs linked per huC242 antibody molecule.

Figure 5 Clearance of 125I-huC242 antibody (F) and 125I-huC242-DM1 conjugate (E) from the blood of mice that had been injected intravenously either with 4.16 mg/kg 125I-huC242 or 4.16 mg/kg 125I-huC242-DM1 conjugate that had, on the average, 4.07 DM1 drugs linked per huC242 antibody molecule.

Each data point is the mean S.D. of the measurements of three samples from three different animals.

Xie, H., Audette, C., Hoffee, M., Lambert, J. M., & Blättler, W. A. (2004). Pharmacokinetics and biodistribution of the antitumor immunoconjugate, cantuzumab mertansine (huC242-DM1), and its two components in mice. Journal of Pharmacology and Experimental Therapeutics, 308(3), 1073-1082.


Specifications

  • Immunogen
  • Synthetic peptides conjugated with KLH and mixed with complete Freund`s adjuvant
  • Host Species
  • Human
  • Derivation
  • Humanized (from mouse)
  • Type
  • Humanized (from mouse) IgG1, κ
  • Specificity
  • Human MUC1
  • Species Reactivity
  • Human
  • Clone
  • Cantuzumab
  • Applications
  • IP, IF, FuncS, FC, Neut, ELISA, ICC

Product Property

  • Purification
  • Protein A/G Purified
  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Immunoprecipitation, Immunofluorescence, Functional Assay, Flow Cytometry, Neutralization, Enzyme-linked Immunosorbent Assay, Immunocytochemistry.

Target

  • Alternative Names
  • EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; mucin-1; H23 antigen; breast carcinoma-associated antigen DF3; cancer antigen 15-3; carcinoma-associated mucin; episialin; krebs von den Lungen-6; mucin 1, transmembrane; peanut-reactive urinary mucin; polymorphic epithelial mucin; tumor associated epithelial mucin; tumor-associated epithelial membrane antigen

Related Resources

  • Biosimilar Overview
Please refer to Cantuzumab Mertansine Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Cantuzumab Mertansine.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone Cantuzumab"

See other products for "MUC1"

Immunotoxin

CAT Product Name Application Type
AGTO-L036E anti-MUC1 immunotoxin C242 (Fab)-PE Cytotoxicity assay, Functional assay
AGTO-L064E anti-MUC1 immunotoxin H23 (scFv)-PE Cytotoxicity assay, Functional assay

Human Antibody

CAT Product Name Application Type
TAB-431MZ Anti-Human MUC1 Recombinant Antibody (PH1) ELISA, WB, FC, IHC, SPR Human antibody
TAB-432MZ Human Anti-MUC1 Recombinant Antibody (TAB-432MZ) ELISA, FC, FACS, DB, SPR Human IgG
TAB-433MZ Human Anti-MUC1 Recombinant Antibody (TAB-433MZ) ELISA, FC, FACS, DB, SPR Human IgG
TAB-434MZ Human Anti-MUC1 Recombinant Antibody (TAB-434MZ) ELISA, FC, FACS, DB, SPR Human IgG
PABX-143-S (P) Recombinant Human Anti-MUC1 Antibody scFv Fragment (CTM01) WB, ELISA, FuncS scFv

Fab Glycosylation

CAT Product Name Application Type
Gly-012LC Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) ELISA, FC, IHC Humanized antibody
Gly-120LC Recombinant Anti-Human MUC1 Antibody (Fab glycosylation) ELISA Mouse antibody

Sialylated IgG Glycan

CAT Product Name Application Type
Gly-012LC-1 Recombinant Anti-Human MUC1 Antibody (Fab glycosylation/Sialylated) ELISA, FC, IHC Humanized antibody

Fc Glycosylation

CAT Product Name Application Type
Gly-109LC Recombinant Anti-Human MUC1 Antibody (Fc glycosylation) ELISA Human antibody

Deglycosylated Antibody (Non-glycosylated IgGs)

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0379MZ Chicken Anti-MUC1 Polyclonal IgY IHC, WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2320 Hi-Affi™ Recombinant Rabbit Anti-MUC1 Monoclonal Antibody (DS2320AB) IHC-P, IHC-Fr IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-H56 Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Pemtumomab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC ADCC enhanced antibody
AFC-TAB-H65 Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Sontuzumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF ADCC enhanced antibody
AFC-TAB-026ML Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Gatipotuzumab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-166 Afuco™ Anti-MUC1 ADCC Recombinant Antibody (Cantuzumab), ADCC Enhanced IP, IF, FuncS, FC, Neut, ELISA ADCC enhanced antibody
AFC-TAB-H77 Afuco™ Human Anti-MUC1 Recombinant Antibody, ADCC Enhanced (AFC-TAB-H77) ELISA, FC, IP, FuncS, IF IgG1, κ

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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