Mouse Anti-N-type blood group antigen Recombinant Antibody (clone NNA7) (CAT#: PABC-096)
Recombinant Mouse Antibody (NNA7) is capable of binding to N-type blood group antigen.
Specific Inquiry
WB
Figure 1 Western blotting of the purified NNA7-derived F(ab).
The purified proteins (3 μg) were loaded onto a 10-percent polyacrylamide gel in the presence of 2-ME and were separated by electrophoresis and transferred to nitrocellulose paper. The blots were then incubated with rabbit anti-mouse F(ab) conjugated with alkaline phospha-tase (which primarily recognizes mouse light chains).
Czerwinski, M., Krop‐Watorek, A., Lisowska, E., & Spitalnik, S. L. (2002). Construction of dimeric F (ab) useful in blood group serology. Transfusion, 42(2), 257-264.
ELISA
Figure 2 Binding of MoAbs and F(ab) to GPA.
Microtiter plate wells were coated with N-type GPA and were analyzed by ELISA at pH 8.3.
Czerwinski, M., Krop‐Watorek, A., Lisowska, E., & Spitalnik, S. L. (2002). Construction of dimeric F (ab) useful in blood group serology. Transfusion, 42(2), 257-264.
Specifications
- Immunogen
- N-type blood group antigen
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Clone
- NNA7
- Applications
- WB, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot and Enzyme-Linked Immunosorbent Assay.
Target
- Alternative Names
- N-type blood group antigen
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone NNA7"
See other products for "N-type Blood Group Antigen"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-717 | Recombinant Anti-N-type Blood Group Antigen Antibody | ELISA, WB, FC, FuncS | IgG |
| MHH-717 | Recombinant Human Anti-N-type Blood Group Antigen Antibody | ELISA, WB, IP, FuncS | IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-717-F(E) | Recombinant Anti-N-type Blood Group Antigen Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| MHH-717-F(E) | Recombinant Human Anti-N-type Blood Group Antigen Antibody Fab Fragment | Neut, IF, FuncS | Fab |
| PFBC-096 | Mouse Anti-N-type blood group antigen Recombinant Antibody (clone NNA7); Fab Fragment | WB, FuncS | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-717-S(P) | Recombinant Anti-N-type Blood Group Antigen Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| MHH-717-S(P) | Recombinant Human Anti-N-type Blood Group Antigen Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| PSBC-096 | Mouse Anti-N-type blood group antigen Recombinant Antibody (clone NNA7); scFv Fragment | WB, FuncS | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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