Human Anti-NECTIN4 Recombinant Antibody (TAB-H25) (CAT#: TAB-H25)

This product is a human IgG1, κ antibody that can recognize human NECTIN4.

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DB

Figure 1 Anti-Human NECTIN4 Therapeutic Antibody (TAB-H25) in DB

Figure 1 Anti-Human NECTIN4 Therapeutic Antibody (TAB-H25) in DB

Dot Blot analysis of TAB-H25 was performed by coating with human NECTIN4 protein (His tag).

TAB-H25 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG was used as a secondary antibody (1: 8000)

WB

Figure 2 Anti-Human NECTIN4 Therapeutic Antibody (TAB-H25) in WB

Figure 2 Anti-Human NECTIN4 Therapeutic Antibody (TAB-H25) in WB

Western blot analysis of TAB-H25 was performed with human NECTIN4 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-H25 and HRP goat anti-Human IgG was used as a secondary antibody (1: 8000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

ELISA

Figure 3 Anti-Human NECTIN4 Therapeutic Antibody (TAB-H25) in ELISA

Figure 3 Anti-Human NECTIN4 Therapeutic Antibody (TAB-H25) in ELISA

ELISA analysis of TAB-H25 was performed by coating with human NECTIN4 protein (His tag). Then blocked with BSA and incubated with TAB-H25. The HRP-conjugated goat anti-Human IgG was used as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

SDS-PAGE

Figure 4 Human Anti-NECTIN4 Recombinant Antibody (TAB-H25, lot#CB1022JF01) in SDS-PAGE

Figure 4 Human Anti-NECTIN4 Recombinant Antibody (TAB-H25, lot#CB1022JF01) in SDS-PAGE

SDS-PAGE analysis of TAB-H25 in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

The purity of TAB-H25 was greater than 95% as determined by SDS-PAGE.

SEC-HPLC

Figure 5 Human Anti-NECTIN4 Recombinant Antibody (TAB-H25, lot#CB1022JF01) in HPLC

Figure 5 Human Anti-NECTIN4 Recombinant Antibody (TAB-H25, lot#CB1022JF01) in HPLC

The purity of TAB-H25 was greater than 95% as determined by SEC-HPLC.

WB

Figure 6 Human Anti-NECTIN4 Recombinant Antibody (TAB-H25, lot#CB1022JF01) in WB

Figure 6 Human Anti-NECTIN4 Recombinant Antibody (TAB-H25, lot#CB1022JF01) in WB

Western blot analysis of TAB-H25 was performed by loading Recombinant NECTIN4 protein.

TAB-H25 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)

ELISA

Figure 7 Human Anti-NECTIN4 Recombinant Antibody (TAB-H25, lot#CB1022JF01) in ELISA

Figure 7 Human Anti-NECTIN4 Recombinant Antibody (TAB-H25, lot#CB1022JF01) in ELISA

ELISA analysis of TAB-H25 was performed by coating with Recombinant Human NECTIN4 Protein. Then blocked with BSA and incubated with Human Anti-NECTIN4 Recombinant Antibody (TAB-H25). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • Human IgG1, κ
  • Specificity
  • Human NECTIN4
  • Species Reactivity
  • Human
  • Applications
  • ELISA, Neut, IF, IP, FC, FuncS

Product Property

  • Purification
  • Protein A/G Purified
  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Neutralization, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay.

Target

  • Alternative Names
  • LNIR; PRR4; EDSS1; PVRL4; nectin-4; nectin-4; Ig superfamily receptor LNIR; nectin 4; poliovirus receptor-related 4; poliovirus receptor-related protein 4

Related Resources

  • Biosimilar Overview
Please refer to Enfortumab Vedotin Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Enfortumab Vedotin.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-H25, RRID: AB_3112028)

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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