Human Anti-NECTIN4 Recombinant Antibody (TAB-H25) (CAT#: TAB-H25)
This product is a human IgG1, κ antibody that can recognize human NECTIN4.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Anti-Human NECTIN4 Therapeutic Antibody (TAB-H25) in DB
Dot Blot analysis of TAB-H25 was performed by coating with human NECTIN4 protein (His tag).
TAB-H25 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG was used as a secondary antibody (1: 8000)
Figure 2 Anti-Human NECTIN4 Therapeutic Antibody (TAB-H25) in WB
Western blot analysis of TAB-H25 was performed with human NECTIN4 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-H25 and HRP goat anti-Human IgG was used as a secondary antibody (1: 8000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 3 Anti-Human NECTIN4 Therapeutic Antibody (TAB-H25) in ELISA
ELISA analysis of TAB-H25 was performed by coating with human NECTIN4 protein (His tag). Then blocked with BSA and incubated with TAB-H25. The HRP-conjugated goat anti-Human IgG was used as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Human Anti-NECTIN4 Recombinant Antibody (TAB-H25, lot#CB1022JF01) in SDS-PAGE
SDS-PAGE analysis of TAB-H25 in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of TAB-H25 was greater than 95% as determined by SDS-PAGE.
Figure 5 Human Anti-NECTIN4 Recombinant Antibody (TAB-H25, lot#CB1022JF01) in HPLC
The purity of TAB-H25 was greater than 95% as determined by SEC-HPLC.
Figure 6 Human Anti-NECTIN4 Recombinant Antibody (TAB-H25, lot#CB1022JF01) in WB
Western blot analysis of TAB-H25 was performed by loading Recombinant NECTIN4 protein.
TAB-H25 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
Figure 7 Human Anti-NECTIN4 Recombinant Antibody (TAB-H25, lot#CB1022JF01) in ELISA
ELISA analysis of TAB-H25 was performed by coating with Recombinant Human NECTIN4 Protein. Then blocked with BSA and incubated with Human Anti-NECTIN4 Recombinant Antibody (TAB-H25). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG1, κ
- Specificity
- Human NECTIN4
- Species Reactivity
- Human
- Applications
- ELISA, Neut, IF, IP, FC, FuncS
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Neutralization, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay.
Target
- Alternative Names
- LNIR; PRR4; EDSS1; PVRL4; nectin-4; nectin-4; Ig superfamily receptor LNIR; nectin 4; poliovirus receptor-related 4; poliovirus receptor-related protein 4
- Gene ID
- 81607
- UniProt ID
- Q96NY8
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NECTIN4"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0034-DJB | Mouse Anti-NECTIN4 Recombinant Antibody (clone N41) | ELISA, IHC, FC | Mouse IgG |
VS4-CJ51 | Recombinant Human Anti-NECTIN4 Antibody (clone 6D3) | ELISA | Human IgG1 |
VS-0723-WK124 | Human Anti-NECTIN4 Recombinant Antibody (VS-0723-WK124) | FC | Chimeric (rabbit/human) IgG1 |
Customer Reviews and Q&As
Excellent for NECTIN4 Detection in Western Blot
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-H25, RRID: AB_3112028)
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For Research Use Only. Not For Clinical Use.
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