Mouse Anti-NELFE Recombinant Antibody (clone 31B3) (CAT#: MOB-3188z)
This product is a mouse antibody that recognizes human NELFE. It can be used in the following applications: WB, IP, IF, ELISA.
Host: Mouse
Isotype: Mouse IgG1, κ
Applications: WB, IP, IF, ELISA
Specific Inquiry
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7594/1220_H3_1_selected.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7594/1220_H3_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46502/799_C12_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/46502/799_C12_1_selected.jpg
Normal Tissue
Figure 3 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7187/21141_A_8_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7187/21141_A_8_8.jpg
Figure 4 Cerebral cortex
(Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7187/21141_B_7_5.jpg
(Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7187/21141_B_7_5.jpg
Figure 5 Liver
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7187/21141_A_7_4.jpg
(Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7187/21141_A_7_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7187/21141_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7187/21141_A_7_5.jpg
Figure 7 Testis
(Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7187/21141_A_6_6.jpg
(Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7187/21141_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204356-NELFE
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204356-NELFE
Specifications
- Immunogen
- Human Negative Elongation Factor Complex Member E
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human NELFE
- Species Reactivity
- Human
- Clone
- 31B3
- Applications
- WB, IP, IF, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Immunoprecipitation Assay, Immunofluorescence Assay, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- NELFE; RD RNA Binding Protein; Major Histocompatibility Complex Gene RD; RDP; RNA-Binding Protein RD; Negative Elongation Factor Complex Member E; Nuclear Protein; Negative Elongation Factor Polypeptide E; Negative Elongation Factor E; RDBP; NELF-E; RD; R
- UniProt ID
- P18615
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
* Abbreviations
AgonistAgonist
BABioassay
InhibInhibition
PGProteogenomics
StimStimulation
WBWestern Blot
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