Mouse Anti-NFE2L2 Recombinant Antibody (clone 14H9)
CAT#: MOB-3994z
This product is a mouse antibody that recognizes human NFE2L2. It can be used in the following applications: IHC, WB, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2990/1586_E11_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43438/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human gallbladder
Immunohistochemical staining of human gallbladder shows cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2990/8743_A_4_4_selected.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_9_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20317/45114_A_4_6.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000116044-NFE2L2
❮
❯
Specifications
- Immunogen
- Human nuclear factor, erythroid 2-like 2
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Antibody Isotype
- IgG1
- Specificity
- Human NFE2L2
- Species Reactivity
- Human
- Clone
- 14H9
- Applications
- IHC, WB, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunohistochemistry Assay, Western Blot, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- NFE2L2; NRF2; Nuclear Factor, Erythroid Derived 2, Like 2; NFE2-Related Factor 2; Nuclear Factor, Erythroid 2-Like 2; Nuclear Factor Erythroid-Derived 2-Like 2; Nuclear Factor (Erythroid-Derived 2)-Like 2; Nuclear Factor Erythroid 2-Related Factor 2
- UniProt ID
- Q16236
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for MOB-3994z. Click the button above to contact us or submit your feedback about this product.
Clear Nuclear Staining In IHC
We used this mouse antibody to detect NFE2L2 in human tissue samples. The clone 14H9 provided excellent results in Immunohistochemistry, showing clear nuclear localization of the transcription factor. The signal-to-noise ratio was high, making it easy to interpret the expression patterns in response to oxidative stress.
Reliable Western Blot Detection
This antibody was effective for Western Blot analysis of cell lysates. It recognized the human NFE2L2 protein specifically. We appreciate that it is a recombinant antibody, as it ensures that we will have a sustainable source of the exact same clone for our future experiments on antioxidant response elements.
Q&As
-
Can this antibody be used for Immunohistochemistry?
A: Yes, this Recombinant Mouse Anti-NFE2L2 Antibody (clone 14H9) is reported for use in Immunohistochemistry (IHC). It can be used on paraffin-embedded sections to detect the nuclear localization of the NFE2L2 transcription factor in human tissues. It is also validated for Western Blot and ELISA.
-
What is the concentration of this antibody?
A: The specific concentration of this antibody is indicated on the vial label of the product you receive. We recommend always referring to the label for the most accurate information to ensure precise calculations for your experimental dilutions and assay setup.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "NFE2L2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1458CT | Recombinant Mouse anti-Human NFE2L2 Monoclonal antibody (2G7) | ELISA, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2432 | Hi-Affi™ Recombinant Rabbit Anti-NFE2L2 Monoclonal Antibody (DS2432AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2085-CN | Rabbit Anti-NFE2L2 Polyclonal Antibody (MRO-2085-CN) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2086-CN | Rabbit Anti-NFE2L2 Polyclonal Antibody (MRO-2086-CN) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2350-CN | Recombinant Rabbit Anti-NFE2L2 (phosphorylated Ser40) Monoclonal Antibody (CBACN-639) | WB, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0014U | Rabbit Anti-NFE2L2 Recombinant Antibody (clone 2D12) | ELISA, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4809 | Anti-NFE2L2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4810 | Anti-Mouse NFE2L2 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
-2.png)
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: WB, ELISA, FuncS
Application: WB, IF, FuncS
Application: FC, IB, Block, Inhib, FuncS, ELISA, FACS, IP, IF
Application: ELISA
Application: FC, FRET, Internalization
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FC, Neut, Inhib
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
