Recombinant Mouse Anti-NGFR Antibody (5H1)
CAT#: NEUT-1779CQ
This antibody is a mouse monoclonal antibody that binds specifically to P75ntr, and it can neutralize the bioactivity of P75ntr.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4765/if_selected.jpg
Normal Tissue
Figure 2 Adrenal gland
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4765/15396_B_6_5_val_selected.jpg
Normal Tissue
Figure 3 Colon
Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1995/5109_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/143/2551_A_9_5.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (RH-30, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000064300-NGFR
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Specifications
- Immunogen
- Human melanoma cell line WM245
- Host Species
- Mouse
- Type
- IgG1
- Specificity
- Recognizes low affinity p75NTR.
- Species Reactivity
- Human, Monkey
- Clone
- 5H1
- Applications
- Used for immunoassay techniques such as: Immunohistochemistry; Immunoprecipitation; Neutralization; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze-thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
| AGTO-L038S | anti-Ngfr immunotoxin MC-192 (IgG)-Sap | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1778CQ | Recombinant Mouse Anti-NGFR Antibody (ME20.4) | EM, ELISA, FC, ICC, IF, IHC-P, Inhib, IP, RIA, WB | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2440 | Hi-Affi™ Recombinant Rabbit Anti-NGFR Monoclonal Antibody (DS2440AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0276CQ-F(E) | Mouse Anti-NGFR Recombinant Antibody (clone MC192); Fab Fragment | ELISA, IHC, IP, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
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| VS3-XY1166 | Mouse Anti-NGFR Recombinant Antibody (clone 2G2) | WB, IP, IF | Mouse IgG1 |
| VS3-WK1900 | Recombinant Rabbit Anti-NGFR Capture Antibody (clone R15-8P-7) | ELISA | Rabbit IgG |
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| VS13-YC806 | Rabbit Anti-NGFR Recombinant Antibody (VS13-YC806) | WB, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR481 | Mouse Anti-NGFR Monoclonal Antibody (VS7-0425-WR481) | WB, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY203 | AbPlus™ Anti-NGFR Magnetic Beads (MC192) | IP, Protein Purification | |
| VS-0724-YC1307 | AbPlus™ Anti-NGFR Magnetic Beads (VS-0724-YC1307) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY341 | Mouse Anti-NHP NGFR Recombinant Antibody (clone NGFR5) | WB, IF, FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC590 | Recombinant Anti-NGFR Vesicular Antibody, EV Displayed (VS-0425-YC590) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4831 | Anti-NGFR Immunohistochemistry Kit | IHC |
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