Afuco™ Anti-Human NONO ADCC Recombinant Antibody (PAT-LM1), ADCC Enhanced
CAT#: AFC-660CL
Anti-NONO ADCC Enhanced Antibody (PAT-LM1) is an ADCC enhanced antibody produced by our Afuco™ platform. PAT-LM1 bingding to non-POU domain-containing octamer-binding protein (NONO) is a natural human antibody that can be used as a potential treatment for multiple types of cancer, with a particularly strong potency against colon, lung, breast, ovary, pancreatic cancers, lympoma.
Published Data
Gene Expression
MTT assay
Figure 4 show a series of graphs of the results of MTT reduction assays for mitochondrial dehydrogenase activity.
The LM-I monoclonal antibody inhibits cell proliferation and decreases survival, or induces apoptosis of EPLC-272H epidermoid cell carcinoma of the lung cells after A) 24 hours of incubation; and B) 48 hours of incubation.
FuncS
Figure 5 shows a graph showing that the LM-I antibody induces apoptosis.
A series of graphs of the results of a cell death ELISA showing that the LM-1 monoclonal antibody induces apoptosis of LOU-NH91 cells after A) 24 hours of incubation; and B) 48 hours of incubation.
Figure 6 The results of screening for antibody LM-I binding to oligosaccharides.
FuncS
Figure 7 shows body weight of LM-I injected mice, which was maintained for 8 weeks post injection.
Body weight in the no injection control and non-specific IgM injected control was reduced by almost 20%, due to poor health from liver metastasis.
Figure 8 shows data indicating that LM-I antibody can reduce tumor metastasis establishment, formation, or proliferation (growth).
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/877_A7_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity and additional strong cytoplasmic staining.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22069/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75% Peripheral nerve/ganglion Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Low Intensity: Weak Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_6_6.jpg
Normal Tissue
Figure 8 Ovary
Follicle cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Ovarian stroma cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_5_7.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147140-NONO
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Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- ADCC enhanced antibody
- Species Reactivity
- Human
- Applications
- WB, ELISA, FC, MTT assay
- Related Disease
- Colon Cancer; Lung Cancer; Breast Cancer; Ovary Cancer; Pancreatic Cancers; Lympoma
Product Property
- Purity
- >95% as judged by SDS-polyacrylamide gel electrophoresis
- Storage
- Store at -20°C. Open under aseptic conditions.
Applications
- Application Notes
- The antibody was validated for Western Blot, ELISA, Flow Cytometry, MTT assay. For details, refer to Published Data.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "NONO"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3007z | Mouse Anti-NONO Recombinant Antibody (clone 24H3) | WB, IF, IHC | Mouse IgG1 |
| MRO-1154-CN | Recombinant Rabbit Anti-NONO Monoclonal Antibody (JG87-38) | WB, IF, IHC | Rabbit IgG |
| ZG-0061U | Rabbit Anti-NONO Recombinant Antibody (clone 8C11) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-FY1048 | Recombinant Rabbit Anti-NONO Antibody (clone R05-1F8) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS13-YC824 | Rabbit Anti-NONO Recombinant Antibody (VS13-YC824) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-538MZ | Anti-Human NONO Recombinant Antibody (PAT-LM1) | WB, SDS-PAGE, ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-538MZ-S(P) | Anti-Human NONO Recombinant Antibody scFv Fragment (PAT-LM1) | WB, SDS-PAGE, ELISA | Human antibody |
| TAB-538MZ-F(E) | Anti-Human NONO Recombinant Antibody Fab Fragment (PAT-LM1) | WB, SDS-PAGE, ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2467 | Hi-Affi™ Recombinant Rabbit Anti-NONO Monoclonal Antibody (DS2467AB) | FC, ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0766-CN-S(P) | Human Anti-NONO Recombinant Antibody; scFv Fragment (HPAB-0766-CN-S(P)) | WB, ELISA, IHC, IP | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0766-CN-F(E) | Human Anti-NONO Recombinant Antibody; Fab Fragment (HPAB-0766-CN-F(E)) | WB, ELISA, IHC, IP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4894 | Anti-NONO Immunohistochemistry Kit | IHC | |
| VS-0525-XY4895 | Anti-Mouse NONO Immunohistochemistry Kit | IHC |
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