Recombinant Mouse Anti-P-glycoprotein Antibody (MRK-16) (CAT#: PABZ-102)

Figure 1 Immunohistochemistry

Immunohistochemical demonstration of P-glycoprotein on untreated lung cancer cells by MRK 16 MAb (A). Negative control (nonimmune mouse serum) was used instead of MRK 16 (B), x 300.

Sugawara, I., Kataoka, I., Morishita, Y., Hamada, H., Tsuruo, T., Itoyama, S., & Mori, S. (1988). Tissue distribution of P-glycoprotein encoded by a multidrug-resistant gene as revealed by a monoclonal antibody, MRK 16. Cancer research, 48(7), 1926-1929.

Figure 2 P-gp expression in Caco-2 cells.

FCM of passage 73, incubated with the external antibody, anti P-gp MRK 16. Shown is the formation of two populations and the overall shift of the positive sample to higher fluoresence intensity as compared to the negative control. MRK 16 NC = negative control.

Anderle, P., Niederer, E., Rubas, W., Hilgendorf, C., Spahn‐Langguth, H., Wunderli‐Allenspach, H., ... & Langguth, P. (1998). P‐glycoprotein (P‐gp) mediated efflux in Caco‐2 cell monolayers: The influence of culturing conditions and drug exposure on P‐gp expression levels. Journal of pharmaceutical sciences, 87(6), 757-762.

Figure 3 Expression of P-gp at the apical membrane of Caco-2 cell monolayers.

Caco-2 cell monolayers were grown for 28 days in culture medium containing vinblastine sulfate (0.011 µM) and processed for CLSM as described in the Experimental Section. The confocal data sets are represented as xy-, xz-, and yz-projections. A, C: labeling for actin (rhodamin−phalloidin) and cell nuclei (Hoechst 33342 stain); B: same data set as A showing the cell nuclei (Hoechst 33342 stain) and the specific labeling with anti P-gp MRK 16; D: same data set as in C showing the labeled nuclei and the labeling with the negative control antibody (MRK 16 negative control). Bar indicates 10 µm.

Anderle, P., Niederer, E., Rubas, W., Hilgendorf, C., Spahn‐Langguth, H., Wunderli‐Allenspach, H., ... & Langguth, P. (1998). P‐glycoprotein (P‐gp) mediated efflux in Caco‐2 cell monolayers: The influence of culturing conditions and drug exposure on P‐gp expression levels. Journal of pharmaceutical sciences, 87(6), 757-762.