Human Anti-PCSK9 Recombinant Antibody (clone J16) (CAT#: PABC-196)

Recombinant Human Antibody (J16) is capable of binding to PCSK9, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-PCSK9 mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-PCSK9 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody, J16, potently inhibit PCSK9 binding to the LDLR extracellular domain and PCSK9-mediated down-regulation of LDLR in vitro.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
IF

Figure 1 PCSK9 internalization with J16.

Figure 1 PCSK9 internalization with J16.

PCSK9-488 internalization in the presence of 5F6 and J16 in HepG2 cells. Internalized human and mouse antibodies shown in blue and red, respectively. Scale bars, 10 μm.

DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4).

IP

Figure 2 APLP2 and LDLR interactions with PCSK9 and their regulation of PCSK9 function.

Figure 2 APLP2 and LDLR interactions with PCSK9 and their regulation of PCSK9 function.

Western blot showing APLP2, PCSK9, orTransferrin receptor (TFNR) levels in input fraction (I), IC or J16 immunoprecipitated samples (IP Ab.) in the absence or presence of 5F6 Fab or 12E3 Fab.

DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4).

IF

Figure 3 Characterization of PCSK9 internalization and subsequent J16 or LDLR lysosomal trafficking in HepG2 cells.

Figure 3 Characterization of PCSK9 internalization and subsequent J16 or LDLR lysosomal trafficking in HepG2 cells.

Colocalization of LDLR (green) with lysosomes (Lamp2; red) in DAPI (blue)-stained cells in the presence of PCSK9IC or PCSK9J16. Scale bars, 10 μm.

DeVay, R. M., Shelton, D. L., & Liang, H. (2013). Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2). Journal of Biological Chemistry, 288(15), 10805-10818.

WB

Figure 4 Identification and biochemical characterization of interactions between PCSK9 and members of the amyloid precursor protein family.

Figure 4 Identification and biochemical characterization of interactions between PCSK9 and members of the amyloid precursor protein family.

Western blots showing APLP2, APP, PCSK9-His, or TFNR levels in input (I), IC IP, or J16 IP samples. co-IPs were performed at pH 6.0 using HepG2 lysates.

DeVay, R. M., Shelton, D. L., & Liang, H. (2013). Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2). Journal of Biological Chemistry, 288(15), 10805-10818.

ELISA

Figure 5 Recombinant PCSK9, APLP2, and LDLR form a trimeric complex in vitro.

Figure 5 Recombinant PCSK9, APLP2, and LDLR form a trimeric complex in vitro.

ELISA of APLP2-PCSK9-LDLR complex with 4 μg/ml PCSK9 and 12 μg/ml IC, J16, or 5F6.

DeVay, R. M., Shelton, D. L., & Liang, H. (2013). Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2). Journal of Biological Chemistry, 288(15), 10805-10818.


Specifications

  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Human PCSK9
  • Species Reactivity
  • Human
  • Clone
  • J16
  • Applications
  • IF, IP, WB, ELISA

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Western Blot, Immunoprecipitation, Immunofluorescence and Enzyme-Linked Immunosorbent Assay.

Target

  • Alternative Names
  • PCSK9; proprotein convertase subtilisin/kexin type 9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3; subtilisin/kexin-like protease PC9; neural apoptosis regulated convertase 1; convertase subtilisin/kexin type 9 preproprotein;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone J16"

See other products for "PCSK9"

Single-domain Antibody

CAT Product Name Application Type
TAB-0080CL-VHH Anti-Human PCSK9 VHH Single Domain Antibody (PKE2) FuncS, Inhib, FC, WB Single domain antibody
TAB-0081CL-VHH Anti-Human PCSK9 VHH Single Domain Antibody (PKF8) FuncS, Inhib, FC, WB Single domain antibody
TAB-0082CL-VHH Anti-Human PCSK9 VHH Single Domain Antibody (PKG1) FuncS, Inhib, FC, WB Single domain antibody
TAB-0083CL-VHH Anti-Human PCSK9 VHH Single Domain Antibody (P1.70) FuncS, Inhib, FC, WB Single domain antibody
TAB-0084CL-VHH Anti-Human PCSK9 VHH Single Domain Antibody (PKE1) FuncS, Inhib, FC, WB Single domain antibody

Neutralizing Antibody

CAT Product Name Application Type
NEUT-1826CQ Recombinant Human Anti-PCSK9 Antibody, Unconjugated Neut IgG1

Rabbit Monoclonal Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-758 Afuco™ Anti-PCSK9 ADCC Recombinant Antibody (Lodelcizumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody
AFC-TAB-H10 Afuco™ Anti-PCSK9 ADCC Recombinant Antibody (Bococizumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS ADCC enhanced antibody
AFC-TAB-H59 Afuco™ Anti-PCSK9 ADCC Recombinant Antibody (Ralpancizumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-H31 Afuco™ Anti-PCSK9 Recombinant Antibody (AFC-TAB-H31), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF Human IgG1, λ

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for PABC-196. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare