Mouse Anti-PLAUR Recombinant Antibody (clone ATN-658) (CAT#: PABL-355)

Figure 1 Immunohistochemical analysis of the inhibition of antibody ATN-658 to the expression of signaling molecules in prostate cancer tumors and skeletal lesions in vivo.

Male Fox chase SCID mice were inoculated with PC-3 cells through the i.t. route of injection, respectively. Animals were treated with 10.0 mg/kg of control IgG (CTL) or ATN-658. At the end of these studies, animals were killed, and tibias were removed, formalin-fixed, and subjected to immunohistochemical analysis as described in Materials and Methods. Result represents the mean ± SEM of five tibias from each group. Significant differences from control are represented by asterisks (P < .05).

Rabbani, S. A., Ateeq, B., Arakelian, A., Valentino, M. L., Shaw, D. E., Dauffenbach, L. M., Kerfoot, C. A., … Mazar, A. P. (2010). An anti-urokinase plasminogen activator receptor antibody (ATN-658) blocks prostate cancer invasion, migration, growth, and experimental skeletal metastasis in vitro and in vivo. Neoplasia (New York, N.Y.), 12(10), 778-88.

Figure 2 Immunohistochemical analysis of the inhibition of antibody ATN-658 to tumor cell proliferation and uPAR expression in vivo.

Male Fox chase SCID mice were inoculated with PC-3 cells through the i.t. route of injection, respectively. Animals were treated with 10.0 mg/kg of control IgG (CTL) or ATN-658. At the end of these studies, animals were killed, and tibias were removed, formalin-fixed, and subjected to immunohistochemical analysis using antibodies against Ki67 and uPAR as described in Materials and Methods. Result represents the mean ± SEM of three tibias from each group. Significant differences from control are represented by asterisks (P < .05).

Rabbani, S. A., Ateeq, B., Arakelian, A., Valentino, M. L., Shaw, D. E., Dauffenbach, L. M., Kerfoot, C. A., … Mazar, A. P. (2010). An anti-urokinase plasminogen activator receptor antibody (ATN-658) blocks prostate cancer invasion, migration, growth, and experimental skeletal metastasis in vitro and in vivo. Neoplasia (New York, N.Y.), 12(10), 778-88.

Figure 1 Recombinant Mouse Anti-uPAR Antibody (ATN-658) (PABL-355) in WB

Western blot analysis of PABL-355 was performed by loading Recombinant Human PLAUR Protein (His Tag).
Lane 1: Non-reducing antigen (0.2μg)
Lane 2: Reducing antigen (1μg)
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)

Figure 2 Recombinant Mouse Anti-uPAR Antibody (ATN-658) (PABL-355) in ELISA

ELISA analysis of PABL-355 was performed by coating with Recombinant Human PLAUR Protein (His Tag).
The secondary antibody: HRP Goat Anti-Mouse IgG (H+L)