Hi-Affi™ Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (TH186DS)
CAT#: MOR-4672
Recombinant Rabbit anti-PRKAA1 monoclonal antibody for WB, IF, ICC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35409/781_E12_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_4.jpg
Normal Tissue
Figure 5 Gallbladder
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64946/144077_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132356-PRKAA1/subcellular
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Specifications
- Immunogen
- Phosphopeptide corresponding to amino acids 168-178 of human AMPK alpha
- Host Species
- Rabbit
- Type
- IgG
- Species Reactivity
- Human
- Clone
- TH186DS
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunocytochemistry
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Applications
- Application Notes
- ICC: 1 µg/ml; IF: 1 µg/ml; WB: 2-5 µg/ml
Target
- Alternative Names
- Protein Kinase AMP-Activated Catalytic Subunit Alpha 1; Protein Kinase, AMP-Activated, Alpha 1 Catalytic Subunit; Hydroxymethylglutaryl-CoA Reductase Kinase; Acetyl-CoA Carboxylase Kinase; Tau-Protein Kinase PRKAA1; AMPK Subunit Alpha-1; ACACA Kinase; HMGCR Kinase; EC 2.7.11.1; AMPK; 5-AMP-Activated Protein Kinase, Catalytic Alpha-1 Chain; AMP-Activated Protein Kinase, Catalytic, Alpha-1; AMP -Activate Kinase Alpha 1 Subunit; AMPK Alpha 1; EC 2.7.11.27; EC 2.7.11.31; EC 2.7.11.26; EC 2.7.11; AMPKa1; Alpha; AMPK1; 1
- Gene ID
- 5562
- UniProt ID
- Q13131
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PRKAA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2844 | Hi-Affi™ Recombinant Rabbit Anti-PRKAA1 Monoclonal Antibody (DS2844AB) | ELISA, IHC-P, WB | IgG |
| MOR-0064-FY | Rabbit Anti-PRKAA1 Recombinant Antibody (clone AFY0035) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0089-CN | Recombinant Mouse Anti-PRKAA1 Monoclonal Antibody (B1-E10) | WB, IF, IHC | Mouse IgG1 |
| ZG-0285F | Mouse Anti-PRKAA1 Recombinant Antibody (ZG-0285F) | WB | Mouse IgG |
| VS3-CJ104 | Mouse Anti-PRKAA1 Recombinant Antibody (VS3-CJ104) | WB, ELISA | Mouse IgG |
| VS3-WK914 | Mouse Anti-PRKAA1 Recombinant Antibody (VS3-WK914) | WB, IHC | Mouse IgG |
| VS3-WK917 | Rabbit Anti-PRKAA1 Recombinant Antibody (VS3-WK917) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY215 | AbPlus™ Anti-PRKAA1 Magnetic Beads (CBACN-025) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC928 | CytoStream™ Rabbit Anti-PRKAA1 Recombinant Antibody (VS13-YC928) | WB, ICC, IF, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1741 | Anti-PRKAA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5671 | Anti-Mouse PRKAA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5670 | Anti-Human PRKAA1 Immunohistochemistry Kit | IHC |
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