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    Hi-Affi™ Recombinant Rabbit Anti-PTK2 Monoclonal Antibody (TH192DS) (CAT#: MOR-4678)

    Recombinant Rabbit anti-PTK2 monoclonal antibody for WB, IF, ICC, IHC-P, FCM, ELISA, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/if_selected.jpg
    Figure 2 IHC staining of human urinary bladder
    Figure 2 IHC staining of human urinary bladder
    (Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_6_5_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Neuronal cells
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Neuropil
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_8_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Hepatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Cells in seminiferous ducts
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_6_6.jpg
    Figure 8 Breast
    Figure 8 Breast
    (Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Myoepithelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_B_1_4.jpg
    Figure 9 Lymph node
    Figure 9 Lymph node
    (Germinal center cells
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous
    Non-germinal center cells
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1842/66971_A_9_8.jpg
    RNA Expression
    Figure 10 RNA cell line category: Low cell line specificity
    Figure 10 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169398-PTK2/subcellular

    Specifications

    • Immunogen
    • APeptide corresponding to amino acids 390-401 of Q05397.
    • Host Species
    • Rabbit
    • Type
    • IgG
    • Species Reactivity
    • Human, Mouse
    • Clone
    • TH192DS
    • Applications
    • Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunocytochemistry; Immunohistochemistry (Paraffin); Flow Cytometry; Enzyme-linked Immunosorbent Assay
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Purified with Absolute Mag™ Protein A Agarose
    • Purity
    • > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
    • Format
    • Liquid
    • Buffer
    • PBS
    • Preservative
    • 0.09% sodium azide
    • Storage
    • Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.

    Applications

    • Application Notes
    • ELISA: 1:500-1:2000; FC: 1-3 µg x 10^6 cells; ICC: 1:500-1:2000; IF: 1-2 µg/ml; IHC-P: 1:10-1:1000; WB: 1:500-1:2000

    Target

    • Alternative Names
    • Protein Tyrosine Kinase 2; Protein Phosphatase 1 Regulatory Subunit 71; Focal Adhesion Kinase-Related Nonkinase; PTK2 Protein Tyrosine Kinase 2; EC 2.7.10.2; Pp125FAK; PPP1R71; P125FAK; FADK 1; FAK1; FRNK; FAK; Protein Phosphatase 1, Regulatory Subunit 71; Focal Adhesion Kinase Isoform FAK-Del33; FAK-Related Non-Kinase Polypeptide; Protein-Tyrosine Kinase 2; Protein Phosphatase 1; Regulatory Subunit 71; EC 2.7.10; FADK

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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