Hi-Affi™ Recombinant Rabbit Anti-PTK2B Monoclonal Antibody (TH195DS)
CAT#: MOR-4681
Recombinant Rabbit anti-PTK2B monoclonal antibody for WB, ChIP, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3850/624_D4_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
Hepatocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_4_6.jpg
Normal Tissue
Figure 8 Tonsil
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Squamous epithelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_6_8.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (Daudi, REH)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000120899-PTK2B/subcellular
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Specifications
- Immunogen
- Peptide with the sequence ESDIAEIPDETL corresponding to amino acids 398-410 of Pyk2
- Host Species
- Rabbit
- Type
- IgG
- Species Reactivity
- Human
- Clone
- TH195DS
- Applications
- Used for immunoassay techniques such as: Western Blot; Chromatin immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Applications
- Application Notes
- ChIP: 1 µl; WB: 4-5 µg/ml
Target
- Alternative Names
- Protein Tyrosine Kinase 2 Beta; Calcium-Regulated Non-Receptor Proline-Rich Tyrosine Kinase; Related Adhesion Focal Tyrosine Kinase; PTK2B Protein Tyrosine Kinase 2 Beta; Calcium-Dependent Tyrosine Kinase; Proline-Rich Tyrosine Kinase 2; Cell Adhesion Kinase Beta; Focal Adhesion Kinase 2; EC 2.7.10.2; CAK-Beta; FADK 2; CADTK; RAFTK; FAK2; CAKB; PYK2; Protein Kinase B; EC 2.7.10; FADK2; PKB; PTK
- Gene ID
- 2185
- UniProt ID
- Q14289
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-1397-FY-F(E) | Mouse Anti-PTK2B Recombinant Antibody; Fab Fragment (HPAB-1397-FY-F(E)) | WB, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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