How effective is this antibody for blocking mouse PD-1 in vitro?

We have designed the rat recombinant anti-Pdcd1 antibody to specifically block mouse PD-1 with high efficiency. The high purity (>95% by SDS-PAGE) and thorough validation in Western blot and blocking assays assure that it produces reproducible results, making it a reliable reagent for in vitro T cell function studies.

Can this antibody be reliably used in immunoblotting and blocking experiments?

Our antibody is thoroughly validated for both Western blot and blocking applications. The rigorous purification process ensures that it delivers consistent results with over 95% purity. The detailed product information provides clear guidelines on usage, making it a dependable tool for investigations into mouse PD-1 functions.

Recombinant Rat Anti-Pdcd1 Antibody (RMP1-14)

CAT#: NEUT-1829CQ

This antibody is a rat monoclonal antibody that binds specifically to mouse PD-1, and it can neutralize the bioactivity of mouse PD-1.

Gene Expression

Normal Tissue Figure 1 Lymph node

Germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35981/161493_A_9_8.jpg

Normal Tissue Figure 2 Tonsil

Germinal center cells
Staining:

High

Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/35981/161493_A_6_8.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, RPMI-8226)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000188389-PDCD1

❮

❯

Specifications

Host Species
Rat

Type
IgG2a

Specificity
Recognizes Mouse PD-1

Species Reactivity
Mouse

Clone
RMP1-14

Applications
Used for immunoassay techniques such as: Blocking; Western Blot

Conjugate
Unconjugated

Product Property

Purity
>95% as determined by analysis by SDS-PAGE

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze-thaw cycles.

Target

Alternative Names
Pdcd1; programmed cell death 1; PD-1; Pdc1; Ly101

Gene ID
18566

UniProt ID
Q02242

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Breast cancer biomarkers at key points during disease progression

Great PD-1 Blocking

The Recombinant Rat Anti-Pdcd1 antibody (RMP1-14) has greatly aided my research in T cell regulation. Its ability to specifically bind and block mouse PD-1 in Western Blot and blocking assays is impressive. High purity and thorough product documentation ensure reliable performance in my immunological experiments.

Consistent and Specific

I have used this anti-Pdcd1 antibody in multiple assays and it has consistently provided specific blocking and detection of mouse PD-1. The detailed technical information and high purity level offer assurance about its performance. The careful documentation of storage and application guidelines has helped maintain its functionality over time.

Q&As

How effective is this antibody for blocking mouse PD-1 in vitro?

A: We have designed the rat recombinant anti-Pdcd1 antibody to specifically block mouse PD-1 with high efficiency. The high purity (>95% by SDS-PAGE) and thorough validation in Western blot and blocking assays assure that it produces reproducible results, making it a reliable reagent for in vitro T cell function studies.
Can this antibody be reliably used in immunoblotting and blocking experiments?

A: Our antibody is thoroughly validated for both Western blot and blocking applications. The rigorous purification process ensures that it delivers consistent results with over 95% purity. The detailed product information provides clear guidelines on usage, making it a dependable tool for investigations into mouse PD-1 functions.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - NEUT-1829CQ.pdf

MSDS

MSDS - NEUT-1829CQ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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