Mouse Anti-PolyQ Tract Recombinant Antibody (clone 3B5H10) (CAT#: PABC-201)

Figure 1 mAb 3B5H10 binds low molecular weight disease-associated polyQ expansions.

3B5H10 preferentially labeled striatal neurons transiently expressing disease-associated polyQ expansions in an exon1 fragment or full-length htt. Scale bar=10 μm.

Miller, J., Arrasate, M., Brooks, E., Libeu, C. P., Legleiter, J., Hatters, D., ... & Widjaja, K. (2011). Identifying polyglutamine protein species in situ that best predict neurodegeneration. Nature chemical biology, 7(12), 925.

Figure 2 3B5H10 recognizes disease-associated polyQ expansions in other neurodegeneration-causing proteins.

HEK293 extracts containing HA epitope–tagged androgen receptor (AR) was blotted with 3B5H10 and α-HA or α-GST antibodies, respectively. 3B5H10 preferentially recognized versions with disease-associated polyQ expansions.

Miller, J., Arrasate, M., Brooks, E., Libeu, C. P., Legleiter, J., Hatters, D., ... & Widjaja, K. (2011). Identifying polyglutamine protein species in situ that best predict neurodegeneration. Nature chemical biology, 7(12), 925.

Figure 3 A species of htt recognized by 3B5H10 best predicts striatal neurodegeneration.

The significance to neurodegeneration of htt species formed in situ and distinguished by 3B5H10 was assessed by Cox analysis with a hierarchical Bayesian statistical approach. Cox coefficient (β) distribution for 3B5H10.

Miller, J., Arrasate, M., Brooks, E., Libeu, C. P., Legleiter, J., Hatters, D., ... & Widjaja, K. (2011). Identifying polyglutamine protein species in situ that best predict neurodegeneration. Nature chemical biology, 7(12), 925.

Figure 4 Epitope recognized by 3B5H10 is preferentially present in low molecular weight htt species.

A solution of Httex1-Q53 was chemically cross-linked and probed with MW7 or 3B5H10. MW7 staining confirms the presence of monomers and small oligomers. The epitope 3B5H10 recognizes is preferentially present in monomers over small oligomers.

Miller, J., Arrasate, M., Brooks, E., Libeu, C. P., Legleiter, J., Hatters, D., ... & Widjaja, K. (2011). Identifying polyglutamine protein species in situ that best predict neurodegeneration. Nature chemical biology, 7(12), 925.

Figure 5 mAb 3B5H10 binds low molecular weight disease-associated polyQ expansions.

Striatal neurons transfected with Httex1-Q46-eGFP was labeled with Alexa 647-conjugated 3B5H10 antibody.

Miller, J., Arrasate, M., Brooks, E., Libeu, C. P., Legleiter, J., Hatters, D., ... & Widjaja, K. (2011). Identifying polyglutamine protein species in situ that best predict neurodegeneration. Nature chemical biology, 7(12), 925.