Mouse Anti-Polysialic Acid Recombinant Antibody (clone mAb735) (CAT#: PABL-309)
Recombinant Mouse Antibody (mAb735) is capable of binding to Polysialic acid.
Specific Inquiry
Figure 1 Western blot analysis of pig embryonic brain homogenate (PEB) and polysialylated NCAM (polySia-NCAM) using mAb735 (0.3 μg/ml), scFv735 (0.13 μg/ml), and scFv735-R55A (0.13 μg/ml). Endo N-treated samples were also analyzed for control experiment.
Nagae, M., Ikeda, A., Hane, M., Hanashima, S., Kitajima, K., Sato, C., & Yamaguchi, Y. (2013). Crystal structure of anti-polysialic acid antibody single chain Fv fragment complexed with octasialic acid insight into the binding preference for polysialic acid. Journal of Biological Chemistry, 288(47), 33784-33796.
Figure 2 ELISA analysis of DP5-PE, DP9-PE, polysialic acid-PE (polySia-PE), and pig embryonic brain homogenate using mAb735 (0.33 μg/ml), scFv735 (3.3 μg/ml), and scFv735-R55A (3.3 μg/ml).
Nagae, M., Ikeda, A., Hane, M., Hanashima, S., Kitajima, K., Sato, C., & Yamaguchi, Y. (2013). Crystal structure of anti-polysialic acid antibody single chain Fv fragment complexed with octasialic acid insight into the binding preference for polysialic acid. Journal of Biological Chemistry, 288(47), 33784-33796.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Polysialic acid
- Clone
- mAb735
- Applications
- WB, IHC, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Polysialic acid
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-787 | Recombinant Anti-polysialic acid Antibody | WB, ELISA, IHC, FuncS | IgG |
| MHH-787 | Recombinant Human Anti-polysialic acid Antibody | WB, FC, FuncS | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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