Mouse Anti-Polysialic Acid Recombinant Antibody (clone mAb735) (CAT#: PABL-309)
Recombinant Mouse Antibody (mAb735) is capable of binding to Polysialic acid.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Figure 1 Western blot analysis of pig embryonic brain homogenate (PEB) and polysialylated NCAM (polySia-NCAM) using mAb735 (0.3 μg/ml), scFv735 (0.13 μg/ml), and scFv735-R55A (0.13 μg/ml). Endo N-treated samples were also analyzed for control experiment.
Nagae, M., Ikeda, A., Hane, M., Hanashima, S., Kitajima, K., Sato, C., & Yamaguchi, Y. (2013). Crystal structure of anti-polysialic acid antibody single chain Fv fragment complexed with octasialic acid insight into the binding preference for polysialic acid. Journal of Biological Chemistry, 288(47), 33784-33796.
Figure 2 ELISA analysis of DP5-PE, DP9-PE, polysialic acid-PE (polySia-PE), and pig embryonic brain homogenate using mAb735 (0.33 μg/ml), scFv735 (3.3 μg/ml), and scFv735-R55A (3.3 μg/ml).
Nagae, M., Ikeda, A., Hane, M., Hanashima, S., Kitajima, K., Sato, C., & Yamaguchi, Y. (2013). Crystal structure of anti-polysialic acid antibody single chain Fv fragment complexed with octasialic acid insight into the binding preference for polysialic acid. Journal of Biological Chemistry, 288(47), 33784-33796.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Polysialic acid
- Clone
- mAb735
- Applications
- WB, IHC, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Polysialic acid
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-787 | Recombinant Anti-polysialic acid Antibody | WB, ELISA, IHC, FuncS | IgG |
| MHH-787 | Recombinant Human Anti-polysialic acid Antibody | WB, FC, FuncS | IgG |
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